Oxygen deprivation inhibits Na+ current in rat hippocampal neurones via protein kinase C

The Journal of Physiology
J P O'ReillyG G Haddad


1. Hippocampal neurones respond to acute oxygen deprivation (hypoxia) with an inhibition of whole-cell Na+ current (INa), although the mechanism of the inhibition is unknown. Kinases can modulate INa and kinases are activated during hypoxia. We hypothesized that kinase activation may play a role in the hypoxia-induced inhibition of INa. 2. Single electrode patch clamp techniques were used in dissociated hippocampal CA1 neurones from the rat. INa was recorded at baseline, during exposure to kinase activators (with and without kinase inhibitors), and during acute hypoxia (with and without kinase inhibitors). 3. Hypoxia (3 min) reduced INa to 38.1 +/- 4.5% of initial values, and shifted steady-state inactivation in the negative direction. Hypoxia produced no effect on activation or fast inactivation. 4. Protein kinase A (PKA) activation with 2.5 mM adenosine 3',5'-cyclic adenosine monophosphate, N6,O2-dibutyryl, sodium salt (db-cAMP) resulted in reduction of INa to 62.8 +/- 5.5% without an effect on activation or steady-state inactivation. INa was also reduced by activation of protein kinase C (PKC) with 5 nM phorbol 12-myristate 13-acetate (PMA; to 40.0 +/- 3.7%) or 50 microM 1-oleoyl-2-acetyl-sn-glycerol (OAG; to 46.1 +/- 2.8%)....Continue Reading


Feb 1, 1992·Pflügers Archiv : European journal of physiology·P G KostyukA S Ter-Markosyan
Sep 1, 1992·Journal of Neuroscience Methods·A R Kay
Jan 1, 1988·The American Journal of Physiology·C Marchetti, A M Brown
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Jun 1, 1989·The American Journal of Physiology·K OnoM Arita
Feb 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M Erecińska, I A Silver
May 1, 1986·Journal of Neuroscience Methods·A R Kay, R K Wong
Sep 1, 1984·Cellular and Molecular Neurobiology·M R Costa, W A Catterall
Nov 30, 1984·Biochemical and Biophysical Research Communications·S Kawamoto, H Hidaka
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Nov 1, 1981·Stroke; a Journal of Cerebral Circulation·J AstrupH R Sørensen
Jun 1, 1994·Progress in Neurobiology·H Meves
Jun 1, 1994·The Journal of Membrane Biology·C M Godoy, S Cukierman
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·C Jiang, G G Haddad
Feb 1, 1993·The Journal of Clinical Investigation·T R CumminsG G Haddad
Jun 1, 1996·The Journal of Membrane Biology·S Cukierman

❮ Previous
Next ❯


Jun 5, 2013·Archives of Pharmacal Research·Jihyun NohJun-Mo Chung
Apr 20, 2001·Brain Research. Molecular Brain Research·N J WebsterC Peers
Apr 14, 2000·Brain Research. Molecular Brain Research·Y XiaG G Haddad
Aug 20, 2015·Biomolecules·Anton HermannThomas M Weiger
Jun 30, 2015·Scientific Reports·Orsolya K PenzErik Keimpema
Oct 27, 2004·Diabetes·Jens R NyengaardJoseph R Williamson
Apr 24, 1999·The European Journal of Neuroscience·S L Mironov, D W Richter
Jul 20, 2000·Clinical and Experimental Pharmacology & Physiology·M L Fung
Jan 5, 2007·Journal of Neurophysiology·Xiang Q GuGabriel G Haddad
Apr 5, 2003·American Journal of Physiology. Cell Physiology·Xiang Q Gu, Gabriel G Haddad
Apr 30, 2004·American Journal of Physiology. Cell Physiology·Xiang Q GuGabriel G Haddad
Jan 11, 2003·Journal of Neurophysiology·Ying XiaGabriel G Haddad
Jun 27, 2003·Journal of Neurosurgical Anesthesiology·Morten C MoeMorten L Vinje
Aug 18, 2001·Journal of Applied Physiology·X Q Gu, G G Haddad
Jul 10, 2001·American Journal of Physiology. Cell Physiology·X Q GuG G Haddad

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.