Oxygen free radical scavengers protect rat islet cells from damage by cytokines.

Diabetologia
W SumoskiA Rabinovitch

Abstract

A possible role for oxygen free radicals in mediating the cytotoxic effects of cytokines in islets was sought by the use of agents that scavenge free radicals. Rat islet cell monolayer cultures were incubated for 6 days with t-butylhydroperoxide, alloxan, streptozotocin, or the cytokines, interleukin 1, tumour necrosis factor, and interferon gamma, without and together with the oxygen free radical scavenger combination of dimethylthiourea and citiolone, and islet cell lysis was measured in a 51chromium cytotoxicity assay. The free radical scavengers significantly inhibited the islet cell cytotoxic effects of t-butylhydroperoxide and alloxan, but not streptozotocin. Similarly, the cytotoxic effects of the cytokine combinations of interleukin 1 plus tumour necrosis factor, interferon gamma plus tumour necrosis factor, and interferon gamma plus interleukin 1 were significantly inhibited by the free radical scavenger combination of dimethylthiourea and citiolone. These results suggest that the cytokine products of macrophages and lymphocytes infiltrating islets in Type 1 (insulin-dependent) diabetes may contribute to B-cell damage by inducing the production of oxygen free radicals in the islet cells.

Citations

Sep 1, 1996·Diabetologia·T Mandrup-Poulsen
Sep 1, 1994·Diabetologia·J NerupT Lorenzen
Jan 1, 1997·International Journal of Clinical & Laboratory Research·M G CavalloP Pozzilli
Dec 1, 1995·Diabetes Research and Clinical Practice·G PapaccioF A Pisanti
Apr 1, 1993·Free Radical Biology & Medicine·J G CorneliusA B Peck
Jan 8, 1999·Neurobiology of Aging·E O'Donnell, M A Lynch
Apr 1, 1990·Journal of Autoimmunity·H Baquerizo, A Rabinovitch
Mar 30, 1999·Journal of Internal Medicine·P KnektA Aromaa
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Y WangK J Lafferty
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·H M KubischJ P Phillips
Dec 23, 2003·Immunopharmacology and Immunotoxicology·Kang-Beom KwonByung-Hyun Park
Apr 25, 2007·Diabetes, Obesity & Metabolism·Kathrin Maedler
May 1, 1994·The Journal of Clinical Investigation·D L EizirikC Hellerström
Feb 3, 2007·Biological & Pharmaceutical Bulletin·Eun Kyung KimJeong Yeh Yang
Jul 28, 2010·Experimental & Molecular Medicine·Mi-Young SongByung-Hyun Park
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·C H SorliR P Robertson
May 26, 2001·Biochemical and Biophysical Research Communications·T TakedaY Seino
Jul 1, 1994·Molecular and Cellular Endocrinology·J Fernández-AlvarezR Gomis
Jul 7, 2009·Biochemical and Biophysical Research Communications·Na LvByung-Hyun Park
Aug 1, 1991·Diabetes Research and Clinical Practice·G Papaccio
Aug 27, 1998·Biochemical Pharmacology·A Rabinovitch, W L Suarez-Pinzon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.