Oxygen inactivation and recovery of nitrogenase activity in cyanobacteria.

Journal of Bacteriology
P T PienkosF R Tabita

Abstract

Exposure of nitrogen-fixing cultures of Anabaena spp. to 100% oxygen resulted in the rapid decline of nitrogenase activity. When oxygen-treated cells were transferred to 100% argon, nitrogenase activity was quickly restored in a process that required protein synthesis. Anaerobiosis was not essential for the recovery process; in fact, cells of Anabaena sp. strains CA and 1F will recover nitrogenase activity after prolonged incubation in 100% oxygen. Oxygen treatment acted directly on the intracellular nitrogenase and did not affect other metabolic processes. Examination of crude extracts of oxygen-treated Anabaena sp. strain CA indicated that both components of nitrogenase are inactivated. However, several lines of evidence suggest that oxygen treatment does not result in irreversible denaturation of nitrogenase, but rather results in a reversible inactivation which may serve as a protection mechanism. Nitrogenase present in crude extracts from cells of Anabaena sp. strain 1F which had been incubated for a prolonged period in 100% oxygen was less sensitive to oxygen in vitro than was nitrogenase of a crude extract of untreated cells.

References

Nov 1, 1978·Journal of Bacteriology·J F Haury, C P Wolk
Jan 1, 1979·Journal of Bacteriology·G StaceyF R Tabita
Jul 1, 1979·European Journal of Biochemistry·P C Hallenbeck Benemann
Jul 26, 1977·Archives of Microbiology·H BotheG Eisbrenner
Aug 1, 1976·European Journal of Biochemistry·A BinderM Avron
Jul 1, 1977·European Journal of Biochemistry·H Haaker, C Veeger
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Jun 28, 1974·Nature·R R Eady, J R Postgate
Dec 23, 1969·Biochimica Et Biophysica Acta·D V DervartanianH Beinert
Jan 1, 1968·Canadian Journal of Microbiology·G W Strandberg, P W Wilson
Jan 1, 1980·Annual Review of Microbiology·R L Robson, J R Postgate
Nov 1, 1980·Archives of Biochemistry and Biophysics·G R Lambert, G D Smith
Jan 1, 1981·Journal of Bacteriology·P T PienkosW J Brill
May 20, 1960·Biochimica Et Biophysica Acta·M AVRON

❮ Previous
Next ❯

Citations

Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
Apr 1, 1988·FEMS Microbiology Reviews·S Hill
Mar 1, 1984·Applied and Environmental Microbiology·M A MurryJ R Benemann
Jun 1, 1992·Microbiological Reviews·P Fay
Feb 1, 1990·Applied and Environmental Microbiology·T A StorchM L Ostrofsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.