PMID: 11311855Apr 20, 2001Paper

Oxygen modulates the release of urokinase and plasminogen activator inhibitor-1 by retinal pigment epithelial cells

The International Journal of Biochemistry & Cell Biology
J T ErichsenM Boulton

Abstract

The aims of this study were to examine the effect of oxygen, in the presence or absence of exogenous growth factors, on the release of plasminogen activators and plasminogen activator inhibitor-1 by cultured human retinal pigment epithelial cells. Antigen and activity levels of urokinase, tissue plasminogen activator and plasminogen activator inhibitor were measured in conditioned media after cells were exposed to three different oxygen environments: hypoxia, normoxia and hyperoxia. Overall proteolytic balance was determined by zymography. The effects of exogenous basic fibroblast growth factor and transforming growth factor-beta were also examined. it was found that retinal pigment epithelial cells released urokinase, tissue plasminogen activator and plasminogen activator inhibitor in measurable quantities. After 48 h, urokinase levels were highest at normoxia, reaching 7.2ng/10(6) cells (+/-2.0 SEM), whereas plasminogen activator inhibitor 1 levels were highest at hyperoxia, reaching 67.5ng/10(6) cells (+/-3.7 SEM). Tissue plasminogen activator levels were minimal (<0.5ng/10(6) cells) and unaffected by both oxygen and growth factors. Overall proteolytic activity was also greatest at normoxia. Fibroblast growth factor stimulat...Continue Reading

References

Jul 1, 1978·The Journal of Experimental Medicine·A Granelli-Piperno, E Reich
Jul 1, 1975·American Journal of Ophthalmology·R Machemer, H Laqua
Oct 1, 1975·American Journal of Ophthalmology·H Laqua, R Machemer
Nov 1, 1992·Current Eye Research·S R PlanckD J Wilson
Dec 4, 1992·Annals of the New York Academy of Sciences·D Collen, H R Lijnen
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·D B RifkinP Mignatti
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M S Pepper, R Montesano
Sep 1, 1991·The Journal of Clinical Investigation·S KourembanasD V Faller
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A HamiltonA K Royston
Oct 1, 1991·The Journal of Investigative Dermatology·V FalangaM B Sporn
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·F BlasiM Ploug
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·E F Plow, L A Miles
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·A VaheriH Tapiovaara
Nov 12, 1985·Biochimica Et Biophysica Acta·O Saksela
Feb 1, 1987·The Journal of Experimental Medicine·A WohlwendJ D Vassalli
May 1, 1989·The Journal of Clinical Investigation·T B ConnorM Lansing
Aug 1, 1994·The British Journal of Ophthalmology·P MoriartyD McLeod
Apr 1, 1995·Experimental Eye Research·A KhaliqM Boulton
Jan 21, 1995·BMJ : British Medical Journal·J M Bland, D G Altman
Aug 1, 1994·Experimental Eye Research·M BoultonP Moriarty
Aug 1, 1994·Current Eye Research·R OsuskyS J Ryan
Dec 15, 1996·The Journal of Clinical Investigation·C BarazzoneA P Sappino
Jan 1, 1997·The International Journal of Biochemistry & Cell Biology·B CullenJ C Geesin
Mar 3, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G FibbiM Del Rosso
Sep 10, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·S F YanD J Pinsky

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Citations

Apr 28, 2004·Microbes and Infection·Kathleen L Collins
Jan 18, 2003·The International Journal of Biochemistry & Cell Biology·Zhiyu Wang, Michelle Kurpakus-Wheater
Aug 1, 2007·Free Radical Biology & Medicine·Jeffrey S ShenbergerAaron Barchowsky
Oct 17, 2009·BJOG : an International Journal of Obstetrics and Gynaecology·M M Binda, P R Koninckx
Sep 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·S Buckley, D Warburton

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