Oxygen-permeable microwell device maintains islet mass and integrity during shipping

Endocrine Connections
Darling M Rojas-CanalesP T Coates

Abstract

Islet transplantation is currently the only minimally invasive therapy available for patients with type 1 diabetes that can lead to insulin independence; however, it is limited to only a small number of patients. Although clinical procedures have improved in the isolation and culture of islets, a large number of islets are still lost in the pre-transplant period, limiting the success of this treatment. Moreover, current practice includes islets being prepared at specialized centers, which are sometimes remote to the transplant location. Thus, a critical point of intervention to maintain the quality and quantity of isolated islets is during transportation between isolation centers and the transplanting hospitals, during which 20-40% of functional islets can be lost. The current study investigated the use of an oxygen-permeable PDMS microwell device for long-distance transportation of isolated islets. We demonstrate that the microwell device protected islets from aggregation during transport, maintaining viability and average islet size during shipping.

References

Apr 1, 1988·Diabetes·C RicordiD W Scharp
Aug 1, 1994·Endocrine Reviews·J F Bach
Aug 1, 1994·Digestive Diseases and Sciences·C Sategna-GuidettiQ Carta
Sep 30, 1993·The New England Journal of Medicine·UNKNOWN Diabetes Control and Complications Trial Research GroupC Siebert
Jun 1, 1996·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H McDevittR Tisch
Nov 30, 2004·Cell Transplantation·M J BarnettJ R T Lakey
Nov 22, 2005·Transplantation Proceedings·P HengsterR Margreiter
Nov 23, 2005·Pediatric Clinics of North America·Michael J HallerDesmond Schatz
Nov 24, 2005·American Journal of Physiology. Endocrinology and Metabolism·R R MacGregorL Stehno-Bittel
Sep 29, 2006·The New England Journal of Medicine·A M James ShapiroJonathan R T Lakey
Jan 19, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K K PapasB J Hering
Mar 1, 2007·Diabetes·Roger LehmannGiatgen A Spinas
Mar 30, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H IchiiC Ricordi
May 15, 2007·Biotechnology and Bioengineering·Klearchos K PapasClark K Colton
Dec 13, 2007·The Journal of Clinical Endocrinology and Metabolism·Lorella MarselliGordon C Weir
Apr 29, 2008·Arquivos brasileiros de endocrinologia e metabologia·George S Eisenbarth, Joy Jeffrey
Jun 21, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Tatsuya KinAndrew Mark James Shapiro
Aug 8, 2008·Current Opinion in Organ Transplantation·Yasuhiro IwanagaKlearchos K Papas
Nov 5, 2010·Diabetes, Obesity & Metabolism·J CantleyD J Withers
Nov 26, 2010·Islets·Timothy J PullenGuy A Rutter
Nov 10, 2011·Current Opinion in Organ Transplantation·A M James Shapiro
Feb 11, 2012·Tissue Engineering. Part C, Methods·Abigail B BernardKristi S Anseth
Mar 13, 2012·Cell Transplantation·Mark J CowleyShane T Grey
May 16, 2012·European Journal of Immunology·Kate L GrahamThomas W H Kay
Mar 16, 2013·Annals of Biomedical Engineering·Dimitrios T HatziavramidisGeorge P Chrousos
May 15, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P J O'ConnellUNKNOWN Australian Islet Transplant Consortium
Jul 31, 2013·Lancet·Mark A AtkinsonAaron W Michels
Oct 26, 2013·Transplantation Proceedings·T-S KimS-K Lee
Dec 3, 2013·Diabetes Research and Clinical Practice·UNKNOWN International Diabetes Federation
Dec 11, 2013·BioMed Research International·Rupert OberhuberMartin Hermann
Apr 30, 2014·ACS Applied Materials & Interfaces·Tianqing LiuBenjamin Thierry

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Citations

Jun 25, 2019·Annals of Plastic Surgery·Lohrasb R SayadiAlan D Widgerow
Nov 25, 2020·Cell Transplantation·Barbara J OlackJoyce C Niland

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Methods Mentioned

BETA
Assay
PCR

Software Mentioned

Elements BR
ImageJ
Elements BR Microsoft
GraphPad Prism
NIS
WVASE32
Solidworks

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