Oxygen radical absorbance capacity of phycocyanin and phycocyanobilin from the food supplement Aphanizomenon flos-aquae

Journal of Medicinal Food
Serena BenedettiFranco Canestrari

Abstract

The oxygen radical absorbance capacity (ORAC) assay has been widely used to quantify peroxyl radical scavenging capacity of pure antioxidant compounds and antioxidant plant/food extracts. However, it has never been applied to natural compounds derived from microalgae-based dietary supplements, namely, phycocyanin (PC) and phycocyanobilin (PCB), for which a strong radical scavenger activity has been documented. In this article, we applied the ORAC method to investigate the capacity of PC and PCB purified from the edible microalga Aphanizomenon flos-aquae to directly quench peroxyl radicals in comparison to well-known antioxidants molecules such as Trolox, ascorbic acid, and reduced glutathione. As a result, PCB was found to have the highest ORAC value (22.18 micromol of Trolox/micromol of compound), comparable to that of PC (20.33 micromol of Trolox/micromol of compound), hence confirming that PCB is mostly responsible for the scavenger activity of PC and making the protein a possible source of the antioxidant in vivo. Our data further corroborate the use of these natural compounds from A. flos-aquae as dietary antioxidant supplements in the treatment of clinical conditions related to oxidative stress.

References

Mar 12, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·C RomayI García
Aug 26, 1998·Biochemical and Biophysical Research Communications·B B VadirajaK M Madyastha
Jan 23, 1999·Methods in Enzymology·G Cao, R L Prior
Jun 1, 2000·Free Radical Biology & Medicine·E A LissiC Romay
Aug 17, 2000·Biochemical and Biophysical Research Communications·V B Bhat, K M Madyastha
May 16, 2001·Biochemical and Biophysical Research Communications·A K PadyanaS Ramakumar
Jul 11, 2001·Biochemical and Biophysical Research Communications·V B Bhat, K M Madyastha
May 12, 2005·Journal of Agricultural and Food Chemistry·Ronald L PriorKaren Schaich
Nov 4, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Serena BenedettiFranco Canestrari

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Citations

Sep 12, 2013·Marine Drugs·Parisa HeydarizadehMartine Bertrand
Apr 3, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Stefano ScoglioSerena Benedetti
Jan 22, 2015·Journal of Agricultural and Food Chemistry·Tugba Boyunegmez TumerCarrie Waterman
Sep 19, 2014·Applied Biochemistry and Biotechnology·Camille JuinLaurent Picot
Feb 24, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Stefano ScoglioSerena Benedetti
Nov 17, 2019·Clinical and Experimental Pharmacology & Physiology·Nancy Pavón-FuentesGiselle Pentón-Rol
Oct 13, 2018·Oxidative Medicine and Cellular Longevity·D NuzzoM Di Carlo

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