PMID: 8938632Oct 1, 1996Paper

Oxygen radical formation, proliferative activity and phagocytic capacity of cultivated macrophages from cirrhotic rat livers

Liver
S VoglR Dargel

Abstract

A method to isolate and cultivate macrophages from Macronodular-cirrhotic rat livers was developed in order to characterize them biochemically, by comparing various functional parameters in macrophage cell cultures from controls and cirrhotic livers. Cells were prepared from female Wistar rats, made cirrhotic by treatment with thioacetamide, by means of a pronase-collagenase digestion method followed by a nycodenz gradient and elutriation. The yield of macrophages was 8.9 x 10(6) cells/g for controls and 10.6 x 10(6) cells/g for cirrhotic livers. The vitality of the cells was > 95%. Forty-eight hours after cultivation, the purity of the cell fractions amounted to 94% and 91% in controls and in the experimental group, respectively. Nitric oxide synthesis was more markedly stimulated by lipopolysaccharide (LPS) in cultures from cirrhotic livers than in those from controls (25 +/- 4 vs 5.8 +/- 1 nmol/10(6) cells/72 hours). Interferon-gamma (IFN-gamma) induced the nitric oxide synthase more rapidly in macrophage cultures from cirrhotic livers than in controls. The production of superoxide anions by macrophages from cirrhotic livers stimulated by zymosan was significantly lower by about 40% when compared with the controls. Incorpora...Continue Reading

References

May 1, 1992·The Journal of Clinical Investigation·C J WiedermannC J Widermann
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T ZimmermannK Jungermann
Aug 1, 1991·European Journal of Clinical Investigation·S Moncada, E A Higgs
Jan 1, 1990·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·S AriiT Tobe
Dec 1, 1989·Liver·Y ShiratoriM Tanaka
Nov 1, 1986·Seminars in Liver Disease·P M HuetA Viallet
Nov 1, 1986·Seminars in Liver Disease·J N Benoit, D N Granger
Mar 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L BouwensE Wisse
Jan 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A RimolaJ Rodes
Apr 18, 1981·British Medical Journal·A HassnerS Shibolet
Sep 1, 1982·Journal of Clinical Pathology·G HoldstockR Wright
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Mar 1, 1994·The Biochemical Journal·R G Knowles, S Moncada
Nov 1, 1994·Journal of Gastroenterology and Hepatology·Y MatsuiY Kamiyama
Nov 15, 1994·Biochemical and Biophysical Research Communications·M ObolenskayaK Decker
Jul 1, 1993·The Journal of Surgical Research·J A Spitzer, A M Mayer

❮ Previous
Next ❯

Citations

Jun 26, 2001·Journal of Gastroenterology and Hepatology·A Ramachandran, K A Balasubramanian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.