Oxygen supply, oxygen consumption, and endocrine and exocrine secretions of the isolated, perfused, porcine pancreas

Acta Physiologica Scandinavica
J J HolstT W Schwartz

Abstract

We studied the effect of varying oxygen supply on the endocrine and exocrine secretions of the isolated perfused porcine pancreas utilizing completely synthetic perfusion medium with and without the addition of erythrocytes. With synthetic medium oxygenated to a Po2 of 500 mmHg, oxygen consumption was constant for flow rates at or above 0.5 ml x min-1 x g-1 (wet weight). Addition of erythrocytes to the medium did not increase oxygen consumption at flow rates above this level. Furthermore, the secretion of fluid, bicarbonate and protein in response to secretin and acetylcholine was not influenced by addition of erythrocytes. Similarly, the secretion of insulin, glucagon, pancreatic polypeptide, and somatostatin in response to arginine and acetylcholine was unchanged; arginine stimulated the secretion of all four peptides, whereas acetylcholine stimulated the secretion of insulin and pancreatic polypeptide and inhibited glucagon and somatostatin secretion. The results indicate that the porcine pancreas is respiring adequately, when perfused with a completely synthetic perfusion medium at flows above about 0.5 ml x min-1 x g-1 and Po2 about 500 mmHg, and that addition of erythrocytes is not necessary for the study of its secretory...Continue Reading

References

Jan 1, 1979·Acta Physiologica Scandinavica·J J HolstJ Fahrenkrug
Dec 1, 1977·Diabetologia·J MarcoM L Villanueva
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Citations

Jan 1, 1989·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·M BarthelW Creutzfeldt
Sep 1, 1986·Journal of the Autonomic Nervous System·J J HolstO V Nielsen
Jun 1, 1982·Regulatory Peptides·L Hilsted, J J Holst
Jul 1, 1985·Regulatory Peptides·F G BaldisseraJ J Holst
Aug 10, 2001·Regulatory Peptides·K TornøeJ J Holst
Jun 17, 2006·Scandinavian Journal of Clinical and Laboratory Investigation·M B DamholtJ Hilsted
Oct 1, 1988·The Journal of Surgical Research·J F Kuemmerle, J M Kellum
May 1, 1982·Digestive Diseases and Sciences·N E PlancheH Sarles
Jun 1, 1997·The Journal of Urology·D P HickeyD M Little
Oct 1, 1990·European Journal of Clinical Investigation·J J Holst
Dec 26, 1996·Annals of the New York Academy of Sciences·K TornøeJ J Holst
Nov 30, 2000·American Journal of Physiology. Endocrinology and Metabolism·K TornøeJ J Holst
May 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·L HansenJ J Holst
Jan 1, 1990·Langenbecks Archiv für Chirurgie·H WaldnerW Brendel
Dec 1, 1983·The American Journal of Physiology·J J HolstO V Nielsen
Oct 1, 1991·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·T MessellJ J Holst
Dec 1, 1995·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·F C BrunicardiD K Andersen

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