Oxygen tension and inhaled nitric oxide modulate pulmonary levels of S-nitrosocysteine and 3-nitrotyrosine in rats

Pediatric Research
Scott A LorchHarry Ischiropoulos

Abstract

The oxidative environment within the lung generated upon administration of oxygen may be a critical regulator for the efficacy of inhaled nitric oxide therapy, possibly as a consequence of changes in nitrosative and nitrative chemistry. Changes in S-nitrosocysteine and 3-nitrotyrosine adducts were therefore evaluated after exposure of rats to 80% or >95% oxygen for 24 or 48 h with and without 20 ppm inhaled nitric oxide. Exposure to 80% oxygen led to increased formation of S-nitrosocysteine and 3-nitrotyrosine adducts in lung tissue that were also associated with increased expression of iNOS. The addition of inhaled nitric oxide in 80% oxygen exposure did not alter any of these adducts in the lung or in the bronchoalveolar lavage (BAL). Exposure to >95% oxygen led to a significant decrease in S-nitrosocysteine and an increase in 3-nitrotyrosine adducts in the lung. Co-administration of inhaled nitric oxide with >95% oxygen prevented the decrease in S-nitrosocysteine levels. The levels of S-nitrosocysteine and 3-nitrotyrosine returned to baseline in a time-dependent fashion after termination of exposure to >95% oxygen and inhaled nitric oxide. These data suggest the formation of S-nitrosating and tyrosine-nitrating species is re...Continue Reading

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Citations

Dec 24, 2005·The New England Journal of Medicine·Mark J D Griffiths, Timothy W Evans
Sep 17, 2005·American Journal of Respiratory Cell and Molecular Biology·David A MunsonHarry Ischiropoulos
Jan 26, 2010·Pediatric Research·Lindsay C JohnstonHarry Ischiropoulos
May 3, 2008·Pulmonary Pharmacology & Therapeutics·Thomas WaldowKlaus Matschke
May 25, 2011·Free Radical Biology & Medicine·Christie J BrunoHarry Ischiropoulos
Oct 28, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Viktoria VereczkiGary Fiskum
Sep 8, 2017·International Journal of Molecular Sciences·Laura Terraneo, Michele Samaja
Jun 1, 2006·Biology of the Neonate·Christiana R FarkouhScott A Lorch
Dec 9, 2020·Pediatric Pulmonology·Vikram BhatiaShyamala Dakshinamurti

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