Oxygen transport by rabbit embryonic blood: high cooperativity of hemoglobin-oxygen binding

Respiration Physiology
R A Holland, S J Calvert


Oxygen equilibrium curves (OECs) have been determined in blood of embryonic and adult rabbits (Oryctolagus cuniculus) using a thin film method (modified Hemoscan). The gestational age of the rabbits was from 12 days to 14 days, the end of the embryonic period. Measurements were made at 37 degrees C and a PCO2 of 21, 42, or 71 mmHg. The most striking finding was an embryonic OEC which was steep above 50% saturation. The Hill plot in this upper region gave a mean nH value of 5.3 in the embryos, the first finding of nH significantly greater than 4 in any normal eutherian mammal. On hemolysis, nH dropped below 4. Oxygen affinity of 14 day embryonic blood was higher than that of adult blood: respective P50 values at PCO2 = 42 mmHg were 29.6 mmHg and 32.5 mmHg. The P50s were somewhat lower at earlier stages. The Bohr effect was measured (as delta log P50/delta log PCO2) in 14 day embryos. Its value was 0.26, about 10% lower than in adults. The results show an apparent aggregation of Hb tetramers, as found in marsupials, but no right shifting of the embryonic OEC compared to the adult OEC.


Oct 28, 1997·European Journal of Biochemistry·R A Holland, A A Gooley
Nov 12, 2005·European Journal of Clinical Investigation·R Baumann, S Dragon
Feb 22, 2011·Proteins·Mitra S Rana, Austen F Riggs
Sep 28, 2002·Anesthesia and Analgesia·Kai-Michael ScheuflerJosef Zentner
Feb 14, 2002·Journal of Applied Physiology·Gösta BerlinRobert D Woodson


Oct 18, 1978·Pflügers Archiv : European journal of physiology·W Jelkmann, C Bauer
Dec 12, 1977·Pflügers Archiv : European journal of physiology·W Jelkmann, C Bauer
Mar 27, 1975·Nature·E R Huehns, A M Faroqui
Oct 23, 1975·Nature·G SteinheiderW Ostertag
May 1, 1987·Pflügers Archiv : European journal of physiology·H Kiwull-SchöneP Kiwull
Jan 1, 1983·Comparative Biochemistry and Physiology. A, Comparative Physiology·R M Wells, T Brittain
Apr 1, 1984·Forensic Science International : Synergy·H H HoppeP J Lincoln
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S J Cooper, R M Hope

Related Concepts

Gestational Age
Chinchilla Rabbits
Oxygen Affinity
Oxygen Binding Activity
Hemoglobin Measurement
Superorder Marsupalia (organism)

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.