Oxygenation of intensive cell-culture system

Applied Microbiology and Biotechnology
A N EmeryMohamed Al-Rubeai

Abstract

The abilities of various methods of oxygenation to meet the demands of high-cell-density culture were investigated using a spin filter perfusion system in a bench-top bioreactor. Oxygen demand at high cell density could not be met by sparging with air inside a spin filter (oxygen transfer values in this condition were comparable with those for surface aeration). Sparging with air outside a spin filter gave adequate oxygen transfer for the support of cell concentrations above 10(7) ml-1 in fully aerobic conditions but the addition of antifoam to control foaming caused blockage of the spinfilter mesh. Bubble-free aeration through immersed silicone tubing with pure oxygen gave similar oxygen transfer rates to that of sparging with air but without the problems of bubble damage and fouling of the spin filter. A supra-optimal level of dissolved oxygen (478% air saturation) inhibited cell growth. However, cells could recover from this stress and reach high density after reduction of the dissolved oxygen level to 50% air saturation.

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Citations

Apr 5, 1997·Biotechnology and Bioengineering·N H SimpsonM Al-Rubeai
Jan 15, 2008·Applied Microbiology and Biotechnology·Kelly Astley, Mohamed Al-Rubeai
Jan 17, 2012·Bioprocess and Biosystems Engineering·Jessica WhelanBrian Glennon
Aug 21, 2013·Current Diabetes Reports·Sophie VériterDenis Dufrane
May 20, 1998·Current Opinion in Biotechnology·M al-Rubeai, R P Singh
Oct 27, 2005·Journal of Biomechanical Engineering·James N WarnockAjit P Yoganathan
Dec 22, 2007·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Jesse P SullivanAndre F Palmer
Aug 5, 2011·Journal of Bioscience and Bioengineering·Kevin MontagneYasuyuki Sakai
Feb 23, 2008·Journal of Biotechnology·Halina SwiderekMohamed Al-Rubeai
Dec 8, 2015·Applied Microbiology and Biotechnology·Benjamin NeunstoecklinMiroslav Soos
Oct 9, 2007·Biotechnology Advances·Era Jain, Ashok Kumar
Sep 13, 2002·Biotechnology and Bioengineering·Kurt BrorsonYuan Xu

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