Abstract
To investigate the potential neuroprotection of oxymatrine in hypoxic-ischemic injury in rat's brain and the associated underlying mechanisms, modified neurological severity scores (mNSS) for neurological functional deficits, 2,3,5-triphenyl-tetrazolium chloride (TTC) staining for infarct volume, TUNEL assay and flow cytometry analysis for apoptosis were assessed. The expressions of Akt, glycogen synthase kinase 3 beta (GSK3β), phosphorylated Akt (p-Akt), phosphorylated GSK3β (p-GSK3β), nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) were measured by western blot. Our results showed that infarct volume and the apoptosis of NeuN-positive cells were significantly reduced in rats that administrated oxymatrine, with a corresponding improvement in neurological function after H/I. Upregulated p-Akt, p-GSK3β, Nrf-2 and HO-1 expressions were observed in response to oxymatrine treatment. Moreover, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 counteracted the protective effect of oxymatrine, evidenced by western blot and histological outcomes. To conclude, our results suggested that oxymatrine could exert efficacious neuroprotective effect against H/I injury by inhibiting apoptosis and oxidati...Continue Reading
References
Jul 28, 2005·Developmental Neuroscience·Robert C Vannucci, Susan J Vannucci
Mar 17, 2009·Brain Research·Ying LiuHong-Guang Fan
Feb 26, 2010·Mediators of Inflammation·Hongguang FanJing Yin
Jul 16, 2010·Neurological Research·Lili CuiWei Du
May 3, 2011·Biological & Pharmaceutical Bulletin·Min LiWei Du
Oct 1, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yong ZhangYong Ding
Jun 25, 2015·Acta Cirúrgica Brasileira·Guanjun JiangTao Qiu
Jun 30, 2015·Neurochemistry International·Peng ZhaoJian-Qiang Yu
Dec 1, 2015·Pharmacology & Therapeutics·Izaskun BuendiaRafael León
Jan 6, 2016·Free Radical Research·Xiao ZhaiXuejun Sun
May 12, 2016·Phytotherapy Research : PTR·Mei-Li LuShi-Hai Xia
Jun 1, 2016·Scientific Reports·Huili ZhuAnding Xu
Apr 11, 2017·Redox Biology·Ulkan KilicErtugrul Kilic
May 4, 2017·The Chinese Journal of Physiology·Shu-Mei ZhaoChun-Yan Guo
Nov 25, 2017·Molecular Neurobiology·Tahir AliMyeong Ok Kim
Citations
May 14, 2020·Natural Product Reports·Donna D Zhang, Eli Chapman
Mar 19, 2019·Oxidative Medicine and Cellular Longevity·Chen-Chen CaiZhen-Lang Lin
May 14, 2020·Molecular Neurobiology·Mohd SalmanSuhel Parvez
Jul 28, 2020·Frontiers in Molecular Neuroscience·Md Abdul HannanIl Soo Moon
Jan 19, 2021·Drug Development Research·Tingjun LiuAnkang Hu
Dec 5, 2020·Oxidative Medicine and Cellular Longevity·Yongpan HuangJiayu Tang
Jan 21, 2021·International Journal of Molecular Sciences·Hadi MohsenpourMohammad Hosein Farzaei
May 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bassant M El-MokademAyman A Soubh
Aug 29, 2021·International Immunopharmacology·Xu LanHao Wang
Feb 1, 2022·Neurochemical Research·Wen-Jun WangJian-Zhong Zhang