Oxymatrine attenuates brain hypoxic-ischemic injury from apoptosis and oxidative stress: role of p-Akt/GSK3β/HO-1/Nrf-2 signaling pathway

Metabolic Brain Disease
Xuhua GeGuoji Zhu

Abstract

To investigate the potential neuroprotection of oxymatrine in hypoxic-ischemic injury in rat's brain and the associated underlying mechanisms, modified neurological severity scores (mNSS) for neurological functional deficits, 2,3,5-triphenyl-tetrazolium chloride (TTC) staining for infarct volume, TUNEL assay and flow cytometry analysis for apoptosis were assessed. The expressions of Akt, glycogen synthase kinase 3 beta (GSK3β), phosphorylated Akt (p-Akt), phosphorylated GSK3β (p-GSK3β), nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) were measured by western blot. Our results showed that infarct volume and the apoptosis of NeuN-positive cells were significantly reduced in rats that administrated oxymatrine, with a corresponding improvement in neurological function after H/I. Upregulated p-Akt, p-GSK3β, Nrf-2 and HO-1 expressions were observed in response to oxymatrine treatment. Moreover, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 counteracted the protective effect of oxymatrine, evidenced by western blot and histological outcomes. To conclude, our results suggested that oxymatrine could exert efficacious neuroprotective effect against H/I injury by inhibiting apoptosis and oxidati...Continue Reading

References

Jul 28, 2005·Developmental Neuroscience·Robert C Vannucci, Susan J Vannucci
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May 12, 2016·Phytotherapy Research : PTR·Mei-Li LuShi-Hai Xia

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Citations

May 14, 2020·Natural Product Reports·Donna D Zhang, Eli Chapman
Mar 19, 2019·Oxidative Medicine and Cellular Longevity·Chen-Chen CaiZhen-Lang Lin
Jan 19, 2021·Drug Development Research·Tingjun LiuAnkang Hu
Jan 21, 2021·International Journal of Molecular Sciences·Hadi MohsenpourMohammad Hosein Farzaei
May 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bassant M El-MokademAyman A Soubh
Aug 29, 2021·International Immunopharmacology·Xu LanHao Wang

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Methods Mentioned

BETA
Protein Assay
electrophoresis
flow cytometry

Software Mentioned

SPSS16
ImageJ
SPSS
Image
Pro Plus

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