Oxymatrine Inhibits Bocavirus MVC Replication, Reduces Viral Gene Expression and Decreases Apoptosis Induced by Viral Infection

Virologica Sinica
Yanqin DingYuning Sun

Abstract

Oxymatrine (OMT), as the main active component of Sophoraflavescens, exhibits a variety of pharmacological properties, including anti-oxidative, anti-inflammatory, anti-tumor, and anti-viral activities, and currently is extensively employed to treat viral hepatitis; however, its effects on parvovirus infection have yet to be reported. In the present study, we investigated the effects of OMT on cell viability, virus DNA replication, viral gene expression, cell cycle, and apoptosis in Walter Reed canine cells/3873D infected with minute virus of canines (MVC). OMT, at concentrations below 4 mmol/L(no cellular toxicity), was found to inhibit MVC DNA replication and reduce viral gene expression at both mRNA and protein levels, which was associated with the inhibition of cell cycle S-phase arrest in early-stage of MVC infection. Furthermore, OMT significantly increased cell viability, decreased MVC-infected cell apoptosis, and reduced the expression of activated caspase 3. Our results suggest that OMT has potential application in combating parvovirus infection.

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Citations

Nov 17, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xu LanFengqin Xu
Aug 29, 2021·International Immunopharmacology·Xu LanHao Wang
Sep 14, 2021·Frontiers in Cellular and Infection Microbiology·Yujie RenXi Zhou

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Methods Mentioned

BETA
transgenic
flow cytometry
PCR
electrophoresis
Assay

Software Mentioned

ModiFit LT
Image J

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