Oxyresveratrol-induced DNA cleavage triggers apoptotic response in Candida albicans

Microbiology
Suhyun Kim, Dong Gun Lee

Abstract

Oxyresveratrol is a naturally occurring phytoalexin produced by plants in response to infection. Biological activities of oxyresveratrol have been studied such as antioxidant, anticancer and anti-inflammation. However, further antimicrobial activity and its mechanism need to be investigated. This study exhibited growth inhibition against pathogenic fungi and investigated its mode of action. Oxyresveratrol inflicted cleavage on DNA, leading to G2/M phase arrest. DNA damage by oxyresveratrol was not the result of oxidative stress but it was triggered by direct binding to DNA. Oxyresveratrol-treated cells showed an apoptotic pathway characterized by phosphatidylserine exposure, apoptotic volume decrease and metacaspase activation. Mitochondria-associated apoptotic features also appeared. Oxyresveratrol-induced Ca2+ overload led to mitochondrial membrane depolarization and release of cytochrome c from mitochondria to cytosol. In conclusion, oxyresveratrol with DNA-binding affinity induces DNA cleavage, and eventually leads to mitochondria-mediated apoptosis in Candida albicans.

References

Apr 4, 2000·Bone Marrow Transplantation·B ErerG Izzi
Jun 17, 2000·Trends in Cell Biology·M J O'ConnellA M Carr
Feb 4, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S S SmailiR J Youle
Nov 6, 2003·Journal of Chemotherapy·F D D'AuriaV Strippoli
Jan 24, 2004·The Journal of Pharmacy and Pharmacology·Kyung-Ook ChungJeon-Ok Moon
Jul 16, 2004·Nucleic Acids Research·Tiffany B SalmonPaul W Doetsch
Nov 24, 2004·Current Opinion in Microbiology·Frank MadeoKai-Uwe Fröhlich
Oct 27, 2005·Bioorganic & Medicinal Chemistry·Kiyoshi FukuharaHaruhiro Okuda
Aug 11, 2006·Trends in Molecular Medicine·Wynand P Roos, Bernd Kaina
Jan 24, 2007·Physiological Reviews·Guido KroemerCatherine Brenner
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Tobias EisenbergFrank Madeo
Sep 7, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C GarzoniJ Garbino
Sep 28, 2007·Acta Microbiologica Et Immunologica Hungarica·Nagendra Nath MishraRandhir Singh
Sep 28, 2007·Inflammation & Allergy Drug Targets·Samarjit Das, Dipak K Das
Aug 5, 2008·BMC Chemical Biology·Maria Hägg OlofssonStig Linder
Jun 11, 2009·Archives of Biochemistry and Biophysics·Mohammad AtharArianna L Kim
Sep 3, 2009·Annals of the New York Academy of Sciences·Ezzatollah KeyhaniFarnoosh Attar
Nov 6, 2009·Cancer Research·Benedicte TrouillerRobert H Schiestl
Jan 16, 2010·Cell Death and Differentiation·D Carmona-GutierrezF Madeo
Jan 26, 2011·Annals of the New York Academy of Sciences·Joseph M Wu, Tze-chen Hsieh
Mar 3, 2011·Genes & Development·Sophie E Polo, Stephen P Jackson
May 4, 2011·The Journal of Microbiology·Joon Kim, Peter Sudbery
Jan 3, 2012·Trends in Plant Science·Ishita AhujaAtle M Bones
Apr 11, 2012·European Journal of Pharmacology·John T WeberJennifer E Slemmer
Aug 25, 2012·Current Biology : CB·Judith Berman
Nov 2, 2012·Antimicrobial Agents and Chemotherapy·Binghua HaoM Hong Nguyen
Apr 30, 2013·International Journal of Food Microbiology·Carme Plumed-FerrerAtte von Wright
Aug 9, 2013·International Journal of Peptide Research and Therapeutics·Agnieszka MarkowskaArkadiusz Surażyński
Oct 3, 2013·PLoS Pathogens·Nicolas PaponRichard J Bennett
Nov 22, 2014·Current Microbiology·Juneyoung Lee, Dong Gun Lee
Feb 14, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sandra Malmgren Hill, Thomas Nyström
Aug 19, 2015·Nature Reviews. Genetics·James B Stewart, Patrick F Chinnery
Oct 7, 2015·Trends in Cell Biology·Katsumori Segawa, Shigekazu Nagata
Jul 28, 2016·Mechanisms of Ageing and Development·Maria AzzopardiRena Balzan

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Citations

Jul 21, 2021·Fungal Biology·Min Seok KwunDong Gun Lee
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kittisak Likhitwitayawuid

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