Oxyresveratrol Induces Autophagy via the ER Stress Signaling Pathway, and Oxyresveratrol-Induced Autophagy Stimulates MUC2 Synthesis in Human Goblet Cells

Antioxidants
Jiah YeomYoung-Hee Lim

Abstract

Autophagy is a cell protection system invoked to eliminate the damaged organelles and misfolded proteins that induce various stresses, including endoplasmic reticulum (ER) stress. Autophagy can control mucin secretion in goblet cells. Oxyresveratrol (OXY), an antioxidant, stimulates expression of MUC2. Thus, we investigated the effect of OXY on autophagy and found that OXY-induced autophagy stimulates MUC2 expression in human intestinal goblet cells. Autophagy-related genes and proteins were examined by quantitative real-time PCR (qPCR) and Western blotting, respectively. Autophagy was assessed by immunocytochemistry (ICC). To analyze the protein expression profiles of OXY-treated LS 174T goblet cells, two-dimensional electrophoresis (2DE) and peptide mass fingerprinting (PMF) were performed. MUC2 expression in cells was evaluated by ICC. OXY significantly increased the expression levels of genes related to autophagy induction, and activated phagosome elongation resulted in the formation of autophagosomes. OXY also activated the ER stress signaling pathway and promoted MUC2 synthesis, which was inhibited by treatment with an autophagy inhibitor. OXY induces autophagy via the ER stress signaling pathway, and OXY-induced autophag...Continue Reading

References

Sep 1, 1993·Journal of Dairy Science·A Bendich
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 27, 2002·European Journal of Clinical Nutrition·M De la Fuente
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Philippe P Roux, John Blenis
Dec 27, 2005·Current Drug Targets. Immune, Endocrine and Metabolic Disorders·B Crimeen-IrwinM Plebanski
Jul 13, 2006·Gastroenterology·Maria Van der SluisAlexandra W C Einerhand
Oct 3, 2006·Nature Cell Biology·Patrice Codogno, Alfred J Meijer
Mar 13, 2010·The Journal of Pathology·Danielle GlickKay F Macleod
Jul 20, 2010·Nature Cell Biology·Brinda RavikumarDavid C Rubinsztein
Aug 18, 2010·Antioxidants & Redox Signaling·Isei Tanida
Dec 29, 2010·Journal of Cell Science·Yongqiang Chen, Daniel J Klionsky
Apr 19, 2011·Genes & Development·Akito TakamuraNoboru Mizushima
Dec 16, 2011·Autophagy·Ellen WirawanPeter Vandenabeele
Mar 28, 2012·Annual Review of Immunology·Petric KuballaRamnik J Xavier
Nov 28, 2012·The Journal of Biological Chemistry·Andriana MargaritiLingfang Zeng
Jan 23, 2013·EMBO Reports·Suresh Subramani, Vivek Malhotra
Feb 2, 2013·Biochimica Et Biophysica Acta·Julie Hollien
Nov 10, 2013·Nature Reviews. Molecular Cell Biology·Christopher A LambSharon A Tooze
Dec 25, 2013·Cell Research·Yuchen FengDaniel J Klionsky
Jul 16, 2014·Inflammatory Bowel Diseases·Malin E V Johansson
Nov 26, 2015·World Journal of Gastrointestinal Oncology·Florin BuradaDan Ionut Gheonea
Feb 24, 2016·Scientific Reports·Dohyun ParkSung Ho Ryu
Sep 3, 2016·Experimental and Therapeutic Medicine·Dae-Ki JoungDong-Yeul Kwon
Nov 7, 2016·Biochimica Et Biophysica Acta. General Subjects·Md Ataur RahmanSung-Oh Huh
Jan 26, 2017·Frontiers in Immunology·Ping KeChong Liu
Mar 18, 2017·Journal of Cell Science·Shuhei Nakamura, Tamotsu Yoshimori
Aug 7, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·HyunA JoYoung-Hee Lim
Aug 29, 2017·International Journal of Molecular Sciences·Saori R Yoshii, Noboru Mizushima
Dec 14, 2017·Essays in Biochemistry·Maria Zachari, Ian G Ganley
May 26, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sarbari SahaSujit K Bhutia
Jun 7, 2018·Frontiers in Neuroscience·Nobuhiro FujikakeShigeomi Shimizu
Jun 24, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Dahyun HwangYoung-Hee Lim

❮ Previous
Next ❯

Citations

May 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiah YeomYoung-Hee Lim
Jun 8, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yating GaoZegeng Li
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kittisak Likhitwitayawuid

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
fluorescence microscopy

Software Mentioned

MASCOT
Statistical Package for the Social Sciences ( SPSS
PDQuset
STRING

Related Concepts

Related Feeds

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Biochemical and Biophysical Research Communications
Takeshi YoshizakiHiroshi Maegawa
Molecular Cancer Therapeutics
Zhineng J YangFrank A Sinicrope
© 2021 Meta ULC. All rights reserved