Oxytocin inhibits LH-stimulated production of androstenedione by bovine theca cells

Molecular and Cellular Endocrinology
M Jo, J E Fortune

Abstract

Oxytocin secretion by bovine granulosa cells increases dramatically after the LH/FSH surge. We have shown that oxytocin stimulates progesterone secretion and inhibits FSH-stimulated estradiol secretion in vitro by granulosa cells from bovine preovulatory follicles obtained before the LH/FSH surge. To determine if oxytocin regulates LH-stimulated steroid production by bovine theca interna cells, theca cells were isolated from preovulatory follicles obtained before the LH surge and were cultured for 4 days in the presence or absence of LH (2 or 4 ng/ml), without or with graded doses of oxytocin (125-1000 ng/ml). LH increased accumulation of androstenedione and progesterone. Oxytocin inhibited LH-stimulated androstenedione production, but had no effect on LH-stimulated progesterone production by cultured theca interna. The next objective was to determine if oxytocin regulates LH-stimulated steroidogenesis by modulating the levels of mRNA for steroidogenic enzymes and/or Steroidogenic Acute Regulatory protein (StAR). Low doses of LH alone increased the levels of mRNA for P450 17 alpha-hydroxylase (17 alpha-OH), 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and cytochrome P450 side-chain cleavage, but not for StAR. In contrast, the...Continue Reading

References

Nov 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A V Sirotkin, J Nitray
Dec 1, 1990·American Journal of Obstetrics and Gynecology·A R FuchsM Fields
Aug 1, 1990·Endocrinology·Y A Chandrasekher, J E Fortune
Nov 1, 1989·Journal of Reproduction and Fertility·D J KiehmD T Armstrong
Sep 1, 1986·Biology of Reproduction·J E Fortune
Nov 1, 1984·Journal of Reproduction and Fertility·S J Dieleman, D M Blankenstein
Feb 5, 1996·Molecular and Cellular Endocrinology·A K BerndtsonJ E Fortune
Mar 1, 1997·Biology of Reproduction·K OkudaT Kimura

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