PMID: 11313432Apr 21, 2001Paper

Oxytocin retrogradely inhibits evoked, but not miniature, EPSCs in the rat supraoptic nucleus: role of N- and P/Q-type calcium channels

The Journal of Physiology
M HirasawaQ J Pittman

Abstract

We previously reported that oxytocin (OXT), released from the dendrites of magnocellular neurons in the supraoptic nucleus (SON), acts retrogradely on presynaptic terminals to inhibit glutamatergic transmission. Here we test the hypothesis that oxytocin reduces calcium influx into the presynaptic terminal. We used nystatin perforated-patch recording in vitro to first identify the calcium channels involved in glutamatergic transmission in the SON. [omega]-Conotoxin GVIA ([omega]-CTx) and [omega]-Agatoxin TK ([omega]-Aga) both reduced evoked EPSC amplitude, while nicardipine and nickel had no effect. A combination of [omega]-CTx and [omega]-Aga completely abolished the evoked EPSCs. This depressant effect was accompanied by an increase in the paired pulse ratio with no change in the kinetics of the evoked EPSCs, AMPA currents or postsynaptic cell properties. These results suggest that presynaptic N- and P/Q-type calcium channels mediate glutamate release in the SON while L-, T- and R-type channels make little or no contribution. Oxytocin-induced reduction of the evoked EPSC was substantially occluded in the presence of [omega]-CTx but only partially in the presence of [omega]-Aga. Amastatin, an endopeptidase inhibitor that increa...Continue Reading

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Citations

Mar 1, 2003·Frontiers in Neuroendocrinology·John A RussellAlison J Douglas
May 15, 2003·Trends in Neurosciences·Mike Ludwig, Quentin J Pittman
Jan 24, 2006·Nature Reviews. Neuroscience·Mike Ludwig, Gareth Leng
Dec 11, 2002·Journal of Neuroendocrinology·J A Russell, G Leng
May 1, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Toshihiko Momiyama
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aleksander R ZampronioQuentin J Pittman
Mar 30, 2010·Frontiers in Neuroendocrinology·Karl J IremongerJaideep S Bains
May 24, 2015·Biological Psychiatry·Valery GrinevichBice Chini
Jun 4, 2005·The European Journal of Neuroscience·Khaleel BhaukaurallyStéphane H R Oliet
Oct 15, 2013·Journal of Neuroendocrinology·V MakaniJ Park
Jan 22, 2003·Journal of Neuroendocrinology·M C Currás-CollazoJ Pandika
Jul 31, 2009·Neuron·Wade G RegehrAaron R Best
Oct 9, 2012·Neuron·Anthony N van den Pol
Jul 31, 2018·Neural Plasticity·Jan BakosZuzana Bacova
Dec 6, 2003·The European Journal of Neuroscience·Tatsushi OnakaKazufumi Honda
Oct 20, 2020·Frontiers in Neuroscience·Erin C McKay, Scott E Counts

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