Oxytocinergic neurons in rat hypothalamus. Dexamethasone-reversible increase in their corticotropin-releasing factor-41-like immunoreactivity in response to osmotic stimulation

Neuroendocrinology
J DohanicsG B Makara

Abstract

Recent results have demonstrated altered corticotropin-releasing factor (CRF)-41 content of the neurointermediate lobe (NIL) of the pituitary gland in response to various manipulations including osmotic stimulation. This study was undertaken to determine whether changes in CRF-41 content of the NIL are accompanied by changes in intensity of CRF-41-like immunoreactivity (CRF-41-LI) of neurosecretory neurones of the hypothalamus in response to osmotic stimulation. Wistar rats of both sexes given either tap water ad libitum, 2% NaCl solution, or access to tap water was limited to 20 min daily, for 7 days. Subsets of rats from each group were adrenalectomized (ADX) or treated with dexamethasone (DEX). Thirty-six hour before perfusion with fixative consisting of buffered formaldehyde and picric acid, animals received 75 micrograms colchicine i.c.v. Forty micrometer thick vibratome sections were stained for CRF-LI, arginine vasopressin (AVP-LI) and oxytocin (OXY-LI) using the avidin-biotin-peroxidase complex method. In response to both types of osmotic stimulation magnocellular neurones of the paraventricular (PVN) and supraoptic nuclei (SON) showed increased CRF-LI, AVP-LI and OXY-LI, while CRF-LI of parvocellular perikarya of the P...Continue Reading

Citations

Dec 29, 1995·Annals of the New York Academy of Sciences·D JezovaM Vigas
Dec 3, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tomás González-HernándezMaría del Carmen González
Jul 22, 1993·Annals of the New York Academy of Sciences·H Raff
Dec 6, 2003·The European Journal of Neuroscience·I H Miklós, K J Kovács
Apr 27, 2005·Endocrinology and Metabolism Clinics of North America·Lauren Jacobson

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