P-element-induced recombination in Drosophila melanogaster: hybrid element insertion.

Genetics
Y H GrayJ A Sved

Abstract

It has previously been shown that the combination of two deleted P elements in trans, one containing the left functional end and the second element the right functional end, can lead to high levels of male recombination. This finding strongly suggests that P-element ends from different chromosomes can become associated, followed by "pseudo-excision". We show that two different processes are involved in resolving the pseudo-excision event: (1) the excised P-element ends continue to function as a single unit (Hybrid Element) and insert at a nearby site in the chromosome or into the element itself [Hybrid Element Insertion (HEI)] and (2) free ends that do not contain P elements repair and rejoin [(Hybrid Excision and Repair (HER)]. Both types of resolution can lead to recombination, and this paper concentrates on the HEI class. One type of HEI event predicts the exact reverse complementary duplication of an 8-bp target site, and we have confirmed the existence of such a structure in six independently derived recombinant chromosomes. There is also a high tendency for insertion events to occur within a few bases of the original 8-bp target site, including six apparent cases of insertion into the exact site.

Citations

Oct 13, 2006·PLoS Genetics·Jianbo ZhangThomas Peterson
Oct 13, 2000·Insect Molecular Biology·D A O'BrochtaM J Lehane
Apr 1, 1998·Trends in Genetics : TIG·G B Gloor, D H Lankenau
May 2, 2006·Genetics·Sandra R SchulzeBarry M Honda
May 17, 2006·Genetics·Dena M Johnson-Schlitz, William R Engels
Apr 25, 2002·Nature Reviews. Genetics·Melissa D Adams, Jeff J Sekelsky
Jul 24, 2007·The Journal of Biological Chemistry·Mei TangDonald C Rio
Feb 26, 2004·Nature Genetics·Annette L ParksHelen L Francis-Lang
Nov 5, 1999·Genetics·J Zhang, T Peterson
Jun 10, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D H Lankenau, G B Gloor
Mar 17, 2001·Genesis : the Journal of Genetics and Development·T ChuS Strickland
Apr 28, 2021·Genetics·Sharu Paul SharmaThomas Peterson
Jun 17, 2000·Science·Y S Rong, K G Golic
Aug 15, 1997·Genes & Development·E L Beall, D C Rio
Mar 21, 2009·Genes & Development·Jianbo ZhangThomas Peterson
May 7, 2009·Genes & Development·Clifford F Weil
Oct 4, 2011·Nucleic Acids Research·Yuan Hu XuanChang-deok Han
Jul 17, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chuanhe YuThomas Peterson

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