P-glycoprotein. Associations between domains and between domains and molecular chaperones.

The Journal of Biological Chemistry
T W Loo, D M Clarke

Abstract

P-glycoprotein consists of two homologous halves, each composed of a transmembrane domain and a nucleotide-binding domain. In order to understand how the domains interact in P-glycoprotein, we expressed each domain as a separate polypeptide and tested for associations using coimmunoprecipitation assays. We found that the interactions between the two halves of P-glycoprotein were mediated through associations between the two transmembrane domains as well as through the nucleotide-binding domains. In addition, the nucleotide-binding domain also associated with the transmembrane domain in each half of the molecule. By contrast, we could not detect any association either between the first nucleotide-binding domain and the second transmembrane domain, or between the second nucleotide-binding domain and the first transmembrane domain. We then tested whether individual domains associated with molecular chaperones, since biogenesis of P-glycoprotein appears to involve the chaperones calnexin and Hsc70. We found that calnexin associated only with the transmembrane domains, while Hsc70 associated only with the nucleotide-binding domains. These results suggest that noncovalent interaction between the domains of P-glycoprotein can contribu...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S E DevineP W Melera
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S V AmbudkarI Pastan
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P GrosF Talbot
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jan 1, 1989·Cell Motility and the Cytoskeleton·K MaruyamaD H MacLennan
Jan 1, 1989·Annual Review of Biochemistry·J A Endicott, V Ling
Jan 1, 1989·Advances in Cancer Research·A M van der Bliek, P Borst
Dec 30, 1987·European Journal of Biochemistry·M ParmentierG Vassart
Jan 13, 1995·The Journal of Biological Chemistry·T W Loo, D M Clarke
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Mar 1, 1994·Trends in Biochemical Sciences·J J BergeronD B Williams
Jan 15, 1994·European Journal of Biochemistry·J Becker, E A Craig
Jan 1, 1993·Advances in Protein Chemistry·D B McKay

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Citations

Jan 1, 1993·Cytotechnology·U A Germann
May 13, 2006·Journal of Bioenergetics and Biomembranes·Tip W LooDavid M Clarke
Jun 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·U A Germann
May 1, 2009·Journal of Medicinal Chemistry·Michael K GannonMichael R Detty
Nov 13, 2008·Cytotechnology·U A Germann, T C Chambers
May 15, 2001·The EMBO Journal·K S Cannon, P Cresswell
Apr 17, 2010·Biochemical and Biophysical Research Communications·Tip W LooDavid M Clarke
Mar 6, 2016·Biochemical and Biophysical Research Communications·Tip W Loo, David M Clarke
Jun 11, 2002·Biochemical and Biophysical Research Communications·Emma HoughAsipu Sivaprasadarao
Jun 30, 2004·Trends in Endocrinology and Metabolism : TEM·Virginie BernierMichel Bouvier
Oct 19, 2006·Biochimica Et Biophysica Acta·Tsutomu ArakawaKouhei Tsumoto
Nov 9, 2004·Biochemical and Biophysical Research Communications·Tip W LooDavid M Clarke
Jul 10, 2013·Biochemical Pharmacology·Tip W LooDavid M Clarke
Apr 14, 2015·BioMed Research International·Arijit BhowmikMrinal Kanti Ghosh
Nov 17, 2004·Trends in Biochemical Sciences·Mark P KrebsShalesh Kaushal
Sep 12, 2002·The Journal of Biological Chemistry·Tip W Loo, David M Clarke
Apr 11, 2007·Physiology·Kenneth J Linton

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