P-glycoprotein, glutathione and glutathione S-transferase increase in a colon carcinoma cell line by colchicine

Journal of Physiology and Biochemistry
M J Ruiz-GómezL Gil

Abstract

The acquisition of resistance to anticancer agents used in chemotherapy is the main cause of treatment failure in malignant disorders, provoking tumours to become resistant during treatment, although they initially respond to it. The main multidrug resistance (MDR) mechanism in tumour cells is the expression of P-gly-coprotein (P-gly), that acts as an ATP-dependent active efflux pump of chemotherapeutic agents. Furthermore, an increased detoxification of compounds mediated by high levels of glutathione (GSH) and glutathione S-transferase (GST), has been found in resistant cells. We developed a study aiming to evaluate the evolution of the main drug resistance markers in tumour cells: P-gly, GSH and GST, during the acquisition of resistance to colchicine, for the purpose of studying the adaptation process and its contribution to the MDR phenomenon. A human colon adenocarcinoma cell line was exposed to colchicine during 82 days, being P-gly, GSH levels and GST activity evaluated by flow cytometry, spectrofluorimetry and spectrophotometry, during exposure time. P-gly and GSH levels increased gradually during the exposure to colchicine, reaching 2.35 and 3.21 fold each. On day 82, GST activity increased 1.84 fold at the end of the ...Continue Reading

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Citations

Jun 22, 2010·The Protein Journal·Berivan Tandogan, N Nuray Ulusu
Mar 21, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Claire VanpouilleNathalie Perek
Jan 24, 2018·Bioscience Reports·Huan-Huan ShaJi-Feng Feng
Jun 28, 2003·Natural Product Reports·Kenneth W Bentley
Sep 28, 2017·International Journal of Molecular Medicine·Hongjun BianJianni Qi
Sep 30, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Francesco FiorentinoAntonello Mai

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