P-selectin blockade ameliorates lupus nephritis in MRL/lpr mice through improving renal hypoxia and evaluation using BOLD-MRI

Journal of Translational Medicine
Liwen ZhangXiao Li

Abstract

Lupus nephritis is one of the most common and severe complications of systemic lupus erythematosus, of which poor prognosis is indicated by aggravated renal hypoxia and tubulointerstitial fibrosis. Cell adhesion molecules play a key role in the progression of lupus nephritis tubulointerstitial lesion, including P-selectin, which mediates the rolling of leukocytes and subsequent adhesion and infiltration and then initiates the inflammatory immune response and ischemia and hypoxia injury. However, the effects and mechanisms of P-selectin in lupus nephritis remain to be investigated, and a noninvasive measurement of lupus nephritis tubulointerstitial hypoxia and fibrosis remains to be explored. Thirty-four MRL/lpr mice were randomly divided into the following three groups: MRL/lpr, saline, and anti-P-selectin, which consisted of no treatment, treatment with normal saline, and treatment with anti-P-selectin monoclonal antibody (mAb) from 12 to 16 weeks of age, respectively. Ten male C57BL/6 mice of the same age served as normal controls. 24-h urinary protein, urinary albumin-creatinine ratio, and periodic acid-Schiff were used to assess kidney damage; Western blot or immunohistochemical staining of the hypoxia probe Hypoxyprobe™-1,...Continue Reading

References

Jan 1, 1985·Advances in Immunology·A N Theofilopoulos, F J Dixon
Mar 9, 1995·The New England Journal of Medicine·M Brezis, S Rosen
May 1, 1994·Kidney International·H R Brady
Nov 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D M StrokaD Candinas
Oct 11, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amanda W WyattGiovanni E Mann
Jun 28, 2003·Trends in Molecular Medicine·Klaus Ley
Mar 9, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gisele HöpflMax Gassmann
Dec 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaodong HeDaniel C Bullard
Mar 6, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Aurora M MorariuRutger J Ploeg
Nov 13, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Renata Polanowska-GrabowskaJoel Linden
May 7, 2011·Clinical and Experimental Pharmacology & Physiology·Fredrik Palm, Lina Nordquist
Mar 18, 2015·Current Pharmaceutical Design·Yong DuChandra Mohan
Jun 30, 2015·Magnetic Resonance Imaging·Stanislas RapacchiDanny J J Wang
Nov 20, 2015·Nature Reviews. Rheumatology·Anne Davidson
Jan 23, 2016·Journal of the American College of Cardiology·Barbara E StähliPhilippe L L'Allier
Jun 25, 2019·Frontiers in Immunology·Heidi HarjunpääSusanna C Fagerholm
Aug 17, 2019·Clinical Journal of the American Society of Nephrology : CJASN·Wei WangZhihong Liu

❮ Previous
Next ❯

Citations

Jul 2, 2021·Science Translational Medicine·Marc ScherlingerPatrick Blanco

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
Assay
ELISA

Software Mentioned

ADW4
Functool

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Zhonghua er ke za zhi. Chinese journal of pediatrics
Jian-ping HuangJi-yun Yang
Clinical Journal of the American Society of Nephrology : CJASN
Giovanna Giannico, Agnes B Fogo
Current Opinion in Nephrology and Hypertension
Glen S Markowitz, Vivette D D'Agati
© 2021 Meta ULC. All rights reserved