P-selectin glycoprotein ligand-1 decameric repeats regulate selectin-dependent rolling under flow conditions.

The Journal of Biological Chemistry
Caroline TauxeOlivier Spertini

Abstract

P-selectin glycoprotein ligand-1 (PSGL-1) interacts with selectins to support leukocyte rolling along vascular wall. L- and P-selectin bind to N-terminal tyrosine sulfate residues and to core-2 O-glycans attached to Thr-57, whereas tyrosine sulfation is not required for E-selectin binding. PSGL-1 extracellular domain contains decameric repeats, which extend L- and P-selectin binding sites far above the plasma membrane. We hypothesized that decamers may play a role in regulating PSGL-1 interactions with selectins. Chinese hamster ovary cells expressing wild-type PSGL-1 or PSGL-1 molecules exhibiting deletion or substitution of decamers with the tandem repeats of platelet glycoprotein Ibalpha were compared in their ability to roll on selectins and to bind soluble L- or P-selectin. Deletion of decamers abrogated soluble L-selectin binding and cell rolling on L-selectin, whereas their substitution partially reversed these diminutions. P-selectin-dependent interactions with PSGL-1 were less affected by decamer deletion. Videomicroscopy analysis showed that decamers are required to stabilize L-selectin-dependent rolling. Importantly, adhesion assays performed on recombinant decamers demonstrated that they directly bind to E-selectin ...Continue Reading

References

Jul 1, 1992·The Journal of Cell Biology·K L MooreR P McEver
Jan 1, 1985·Annual Review of Cell Biology·J L GoldsteinW J Schneider
Oct 1, 1994·Current Opinion in Cell Biology·S D Rosen, C R Bertozzi
Feb 1, 1995·The Journal of Experimental Medicine·K LeyA L Beaudet
Feb 10, 1997·The Journal of Cell Biology·M B LawrenceK Ley
Apr 21, 1997·The Journal of Cell Biology·D J GoetzF W Luscinskas
Sep 28, 1999·The Journal of Experimental Medicine·G M RomoJ A López
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RamachandranR P McEver
Feb 8, 2000·The Journal of Biological Chemistry·A M FongD D Patel
Sep 17, 2002·Current Opinion in Cell Biology·Rodger P McEver
Oct 31, 2002·The Journal of Biological Chemistry·Michael Pierre BernimoulinOlivier Spertini
May 21, 2003·The Journal of Experimental Medicine·Markus SperandioKlaus Ley
Jun 28, 2003·Trends in Molecular Medicine·Klaus Ley
Oct 2, 2003·Current Opinion in Cell Biology·John B Lowe
Oct 24, 2003·The Journal of Biological Chemistry·Krishna K SarangapaniCheng Zhu
Dec 4, 2003·Annals of Human Genetics·D A TregouetUNKNOWN AtheroGene group
Sep 15, 2004·The Journal of Cell Biology·Tadayuki YagoRodger P McEver
Oct 23, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Peter BugertHarald Klüter
Jan 16, 2007·Journal of Thrombosis and Thrombolysis·Beste OzbenAytac Oncul
Sep 18, 2007·BMC Evolutionary Biology·Bénédicte BaïsseOlivier Spertini
Jun 27, 1949·Annals of the New York Academy of Sciences·E J KEPLER

❮ Previous
Next ❯

Citations

Jul 15, 2009·Immunological Reviews·Douglas A CarlowHermann J Ziltener
May 3, 2013·Molecular Nutrition & Food Research·Rafael LlorachCristina Andres-Lacueva
Jul 16, 2019·Journal of Leukocyte Biology·Bénédicte BaïsseOlivier Spertini
Apr 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yajing FuYuntao Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Nature Reviews. Molecular Cell Biology
Cornelia Hahn, Martin A Schwartz
Expert Review of Cardiovascular Therapy
Ioannis PantosDemosthenes Katritsis
Nature Reviews. Cardiology
Kevin J Woollard, Frederic Geissmann
© 2022 Meta ULC. All rights reserved