P-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow

The Journal of Clinical Investigation
Lijun XiaR P McEver

Abstract

P-selectin glycoprotein ligand-1 (PSGL-1) mediates rolling of leukocytes on P-selectin under flow. The glycoproteins that enable leukocyte tethering to or rolling on E-selectin are not known. We used gene targeting to prepare PSGL-1-deficient (PSGL-1-/-) mice, which were healthy but had moderately elevated total blood leukocytes. Fluid-phase E-selectin bound to approximately 70% fewer sites on PSGL-1-/- than PSGL-1+/+ neutrophils. Compared with PSGL-1+/+ leukocytes, significantly fewer PSGL-1-/- leukocytes rolled on E-selectin in vitro, because their initial tethering to E-selectin was impaired. The residual cells that tethered rolled with the same shear resistance and velocities as PSGL-1+/+ leukocytes. Compared with PSGL-1+/+ mice, significantly fewer PSGL-1-/- leukocytes rolled on E-selectin in TNF-alpha-treated venules of cremaster muscle in which P-selectin function was blocked by an mAb. The residual PSGL-1-/- leukocytes that tethered rolled with slow velocities equivalent to those of PSGL-1+/+ leukocytes. These results reveal a novel function for PSGL-1 in tethering leukocytes to E-selectin under flow.

References

Feb 28, 1992·Biochemical and Biophysical Research Communications·C HessionR Lobb
May 1, 1985·Microvascular Research·U NobisP Gaehtgens
Jan 1, 1980·Microvascular Research·G W Schmid-SchönbeinS Chien
Feb 1, 1995·The Journal of Experimental Medicine·K LeyA L Beaudet
Feb 1, 1995·The Journal of Cell Biology·K L MooreR P McEver
Jul 12, 1996·Science·T G DiacovoU H von Andrian
Jan 15, 1997·The Journal of Clinical Investigation·A Varki
Jun 1, 1997·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·U Jung, K Ley
Aug 24, 1999·The Journal of Biological Chemistry·A LeppänenR D Cummings
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S D RobinsonR O Hynes
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RamachandranR P McEver
Jun 13, 2001·The Journal of Cell Biology·C J DimitroffR Sackstein
Jun 19, 2001·The Journal of Biological Chemistry·M K WildD Vestweber
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·V RamachandranR P McEver

❮ Previous
Next ❯

Citations

Jul 6, 2012·Nature·Prithu SunddKlaus Ley
Nov 26, 2008·Nature Medicine·Paolo F FabeneGabriela Constantin
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tadayuki YagoLijun Xia
Jan 12, 2011·The Journal of Biological Chemistry·Jonathan J MinerRodger P McEver
May 21, 2003·The Journal of Experimental Medicine·Markus SperandioKlaus Ley
Oct 7, 2004·The Journal of Experimental Medicine·Michael L SmithKlaus Ley
Apr 13, 2005·The Journal of Experimental Medicine·Yoshio KatayamaPaul S Frenette
Mar 29, 2006·The Journal of Experimental Medicine·Jesús Rivera-NievesKlaus Ley
May 23, 2007·The Journal of Experimental Medicine·Guangyu AnLijun Xia
Dec 15, 2010·The Journal of Experimental Medicine·Zhenghui LiuRodger P McEver
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Rodger P McEver, Cheng Zhu
Sep 18, 2007·BMC Evolutionary Biology·Bénédicte BaïsseOlivier Spertini
Dec 3, 2014·Trends in Molecular Medicine·Stefano Angiari, Gabriela Constantin
Oct 16, 2013·The Journal of Experimental Medicine·Anika StadtmannAlexander Zarbock
Jun 15, 2007·The Journal of Allergy and Clinical Immunology·Margaret KellyPaul Kubes
Apr 5, 2011·The American Journal of Pathology·Maria NácherAndrés Hidalgo
Dec 19, 2017·Acta Neuropathologica·Golo KronenbergKaren Gertz
Jun 19, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·János KappelmayerHendra Setiadi

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
FCS
transfection
flow cytometry

Software Mentioned

CellQuest
PSGL
Inovision
CircuSoft
SPSS
Stat
Sigma

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.