P-type ATPases mediate sodium and potassium effluxes in Schwanniomyces occidentalis.

The Journal of Biological Chemistry
María A Bañuelos, A Rodríguez-Navarro

Abstract

Two genes isolated from Schwanniomyces occidentalis, ENA1 and ENA2, encode P-type ATPases highly homologous to the Na-ATPases of Saccharomyces cerevisiae and complement the Na+ sensitivity of an S. cerevisiae mutant strain lacking its own Na-ATPases. The expression of both ENA1 and ENA2 was highly dependent on a high external pH, but whereas a high pH was sufficient for the expression of ENA2, the expression of ENA1 required a high pH and the presence of Na+. Disruption of ENA1 rendered the cells less tolerant to Na+ than the wild-type strain and decreased their capacity for Na+ extrusion. Disruption of ENA2 did not affect Na+ tolerance, but decreased both the growth at high pH and K+ efflux. We discuss these results and propose that fungal Na-ATPases should be considered alkali cation ATPases. By sequence comparison, we found that fungal Na-ATPases form a homogeneous group that can be distinguished from other cation-pumping P-type ATPases, except from the cta3 Ca-ATPase of Schizosaccharomyces pombe.

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Citations

Sep 17, 2009·Plant Molecular Biology·Ana Fraile-EscancianoBegoña Benito
Jun 10, 2005·FEMS Yeast Research·Catarina PristaJosé Ramos
Feb 29, 2000·Biochimica Et Biophysica Acta·A Rodríguez-Navarro
Oct 20, 2005·Journal of Bioscience and Bioengineering·Y WatanabeY Tamai
Mar 11, 2000·Molecular Microbiology·B BenitoA Rodríguez-Navarro
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Mónica Viejo-DíazJosé F Fierro
Oct 24, 2007·Eukaryotic Cell·Amparo Ruiz, Joaquín Ariño
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Joaquín AriñoHana Sychrová
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kazuya Yoshida
Jul 16, 1999·Critical Reviews in Biotechnology·T T WangB H Lee
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Begoña Benito, Alonso Rodríguez-Navarro
Sep 8, 2018·Yeast·Joaquín AriñoHana Sychrova
Apr 5, 2002·Microbiology·Begoña BenitoAlonso Rodríguez-Navarro
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Gillian L FellAnne G Rosenwald
Aug 10, 2000·Molecular Microbiology·M A BañuelosA Rodríguez-Navarro
Jan 19, 2011·FEMS Microbiology Letters·José RamosHana Sychrová
Sep 2, 2020·Scientific Reports·Ane Markina-IñarrairaeguiEduardo A Espeso
Jul 24, 2010·Biochimica Et Biophysica Acta·Alonso Rodríguez-Navarro, Begoña Benito

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