P120ctn overexpression enhances beta-catenin-E-cadherin binding and down regulates expression of survivin and cyclin D1 in BEL-7404 hepatoma cells

World Journal of Gastroenterology : WJG
Chao-zan NongHua-yi Huang

Abstract

To understand the role of P120ctn in E-cadherin-mediated cell-cell adhesion and signaling as well as in hepatoma cell biological function. We stably overexpressed p120ctn isoform 3A in BEL-7404 human hepatoma cells and studied the effect of p120ctn on beta-catenin and E-cadherin binding as well as p120ctn and beta-catenin subcellular localization using immunoprecipitation, Western blotting and confocal microscopy. We also investigated the inhibitory effect of p120ctn transfection on the expression of apoptotic protein survivin survivin and cell cycle regulator cyclin D1 in the cells. Western blotting indicated that p120ctn expression increased after cells were transfected with p120ctn isoform 3A. The protein was located mainly at membrane under immunofluorescent microscope. Beta-catenin nuclear expression was reduced after overexpression of p120ctn isoform 3A. The p120ctn-E-cadherin binding increased after transfection of p120ctn isoform 3A. Furthermore, overexpression of p120ctn down regulated the expression of apoptotic protein survivin and cell cycle regulator cyclin D1. These effects led to reduction of cell proliferation. Our results indicate that p120ctn plays an important role in regulating the formation of E-cadherin an...Continue Reading

References

Mar 21, 1997·Science·M Peifer
Apr 17, 1999·Molecular and Cellular Biology·J M Daniel, A B Reynolds
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·J van HengelF van Roy
Nov 13, 2002·The Journal of Cell Biology·Renee C IretonAlbert B Reynolds
Dec 21, 2002·Differentiation; Research in Biological Diversity·Molly A Thoreson, Albert B Reynolds
Sep 4, 2003·Journal of Cell Science·Agnes Roczniak-Ferguson, Albert B Reynolds
Dec 17, 2003·The Journal of Biological Chemistry·Masahiro YanagisawaPanos Z Anastasiadis
Mar 23, 2004·Journal of Dermatological Science·Yasuko IshizakiKatsuhiko Enomoto
May 27, 2004·The American Journal of Pathology·Tatsuhiro ShibataSetsuo Hirohashi
Dec 22, 2004·British Journal of Cancer·F Li
Jul 13, 2005·Neuropathology and Applied Neurobiology·J PizemM Popovic
Aug 16, 2005·Current Opinion in Cell Biology·Jack Lilien, Janne Balsamo
Oct 26, 2005·Biochemical and Biophysical Research Communications·Tania YasminToshiyuki Sasaguri

❮ Previous
Next ❯

Citations

Oct 18, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhangjun ChengFeng Shen
Mar 10, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mira TöyliSinikka Eskelinen
Apr 3, 2020·Biochimica Et Biophysica Acta. Biomembranes·Lorenza González-MariscalElida Amaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis