p130Cas-dependent actin remodelling regulates myogenic differentiation

The Biochemical Journal
Keiko KawauchiYasuhiro Sawada

Abstract

Actin dynamics are implicated in various cellular processes, not only through the regulation of cytoskeletal organization, but also via the control of gene expression. In the present study we show that the Src family kinase substrate p130Cas (Cas is Crk-associated substrate) influences actin remodelling and concomitant muscle-specific gene expression, thereby regulating myogenic differentiation. In C2C12 myoblasts, silencing of p130Cas expression by RNA interference impaired F-actin (filamentous actin) formation and nuclear localization of the SRF (serum-response factor) co-activator MAL (megakaryocytic acute leukaemia) following the induction of myogenic differentiation. Consequently, formation of multinucleated myotubes was abolished. Re-introduction of wild-type p130Cas, but not its phosphorylation-defective mutant, into p130Cas-knockdown myoblasts restored F-actin assembly, MAL nuclear localization and myotube formation. Depletion of the adhesion molecule integrin β3, a key regulator of myogenic differentiation as well as actin cytoskeletal organization, attenuated p130Cas phosphorylation and MAL nuclear localization during C2C12 differentiation. Moreover, knockdown of p130Cas led to the activation of the F-actin-severing p...Continue Reading

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Citations

Dec 12, 2012·Genes & Cancer·Hiroyuki MatsuiYasuhiro Sawada
Mar 26, 2014·The Journal of Cell Biology·Shota YamauchiKeiko Kawauchi
Jul 10, 2013·Antioxidants & Redox Signaling·Sai Srinivas Panapakkam Giridharan, Steve Caplan
Jun 26, 2014·IUBMB Life·Anna S NikonovaErica A Golemis
Jan 30, 2014·Biochemical and Biophysical Research Communications·Da Eun JeongWook Kim
Mar 3, 2015·Gene·Maria del Pilar Camacho LealSara Cabodi
Mar 22, 2014·Journal of Cellular Physiology·Alvin Kunyao GuoKeiko Kawauchi
Oct 18, 2013·American Journal of Physiology. Cell Physiology·Kimberly M Stroka, Konstantinos Konstantopoulos
Feb 14, 2017·BioMed Research International·Takahiro EbataKeiko Kawauchi
Mar 25, 2019·Stem Cells International·Rosaria SantoroGiulio Pompilio
Jan 13, 2017·BioMed Research International·Takahiro EbataKeiko Kawauchi
Jun 15, 2014·Journal of Cell Science·Hiroaki MachiyamaYasuhiro Sawada
Mar 15, 2015·Journal of Cell Science·Genevieve S GriffithsMichelle L Matter

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