P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryote cells

Molecular Microbiology
Tracey A BurnettSteven P Djordjevic

Abstract

Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, colonizes the respiratory cilia of affected swine causing significant economic losses to swine production worldwide. Heparin is known to inhibit adherence of M. hyopneumoniae to porcine respiratory epithelial cilia. M. hyopneumoniae cells bind heparin but the identity of the heparin-binding proteins is limited. Proteomic analysis of M. hyopneumoniae lysates identified 27 kDa (P27), 110 kDa (P110) and 52 kDa (P52) proteins representing different regions of a 159 kDa (P159) protein derived from mhp494. These cleavage fragments were surface located and present at all growth stages. Following purification of four recombinant proteins spanning P159 (F1P159, F2P159, F3P159 and F4P159), only F3P159 and F4P159 bound heparin in a dose-dependent manner (K(d) values 142.37 +/- 22.01 nM; 75.37 +/- 7.34 nM respectively). Scanning electron microscopic studies showed M. hyopneumoniae bound intimately to porcine kidney epithelial-like cells (PK15 cells) but these processes were inhibited by excess heparin and F4P159. Similarly, latex beads coated with F2P159 and F4P159 adhered to and entered PK15 cells, but heparin, F2P159 and F4P159 was inhibitory. These findings ind...Continue Reading

References

Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A D Cardin, H J Weintraub
Mar 1, 1995·Infection and Immunity·Q ZhangR F Ross
May 16, 1998·Biochemistry·G J PankhurstS B Easterbrook-Smith
Apr 18, 2000·Infection and Immunity·F C MinionT Hsu
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Apr 25, 2002·Molecular Microbiology·Franco D MenozziCamille Locht
Jun 5, 2002·Frontiers in Bioscience : a Journal and Virtual Library·F Chris Minion
Feb 11, 2003·Microbiology·Luc BelloyJoachim Frey
Dec 5, 2003·Veterinary Microbiology·J ViccaF Haesebrouck
Apr 23, 2004·Infection and Immunity·Steven P DjordjevicF Chris Minion
Oct 19, 2004·Journal of Bacteriology·F Chris MinionGregory G Mahairas
Oct 22, 2005·Infection and Immunity·Cary AdamsF Chris Minion
Dec 22, 2005·Infection and Immunity·Cheryl JenkinsSteven P Djordjevic

❮ Previous
Next ❯

Citations

Aug 13, 2009·Applied Biochemistry and Biotechnology·Yuan-Zuo LiDavid Shiuan
Jan 20, 2011·The Journal of Biological Chemistry·Lisa M SeymourMark J Walker
Dec 12, 2007·Infection and Immunity·Melissa L MadsenF Chris Minion
Jun 8, 2012·The Journal of Veterinary Medical Science·Ping ZhaoZhong-xin Lu
Dec 24, 2013·Veterinary Research·Gareth A MaglennonUNKNOWN BRaDP1T consortium
Jan 16, 2015·Open Biology·Veronica M JarockiSteven P Djordjevic
Apr 2, 2016·Research in Veterinary Science·Haiyan WangGuoqing Shao
Jun 21, 2011·Veterinary Microbiology·G F BrowningP F Markham
Dec 30, 2006·Veterinary Microbiology·D CalusD Maes
May 1, 2010·Microbiology·Stuart W Gardner, F Chris Minion
May 29, 2016·Research in Veterinary Science·Hassan Z A IshagGuo-Qing Shao
May 6, 2015·Veterinary Microbiology·Benjamin B A Raymond, Steven Djordjevic
Oct 22, 2014·PloS One·Franciele Maboni SiqueiraArnaldo Zaha
Apr 8, 2017·Proteomes·Matthew P PadulaSteven P Djordjevic
Aug 24, 2017·Transboundary and Emerging Diseases·D MaesM Pieters
Apr 7, 2018·Molecular Microbiology·Mariana Galvao FerrariniMarie-France Sagot
Feb 13, 2009·Expert Review of Proteomics·Nichollas E Scott, Stuart J Cordwell
Mar 15, 2018·Frontiers in Cellular and Infection Microbiology·Benjamin B A RaymondSteven P Djordjevic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.