p16 methylation does not affect protein expression in cervical carcinogenesis

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
Kristina NehlsNicolas Wentzensen

Abstract

Previous studies have reported a frequency range of 19-61% for p16 methylation in cervical cancers. However, p16 is strongly expressed in over 90% of cervical cancers and pre-cancers, due to interactions of HPV oncogenes with p53 and pRb. In order to clarify these controversial findings, we developed a new bisulphite sequencing protocol to determine the methylation status of p16. DNA extracted from 17 cell lines and 94 microdissected clinical samples was subjected to methylation analysis. p16 expression was confirmed in Western blot and immunohistochemistry. Complete methylation of p16 was found in none of the dysplastic lesions, but in 26% of the cervical carcinomas. However, immunohistochemistry showed strong p16 expression in all cancers. These findings indicate that p16 methylation does not implicate loss of p16 expression in HPV-induced tumours. In cervical cancer, methylation of p16 does not seem to be an underlying pathogenic mechanism, but may be a result of increasing genetic and epigenetic instability.

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Citations

Jun 16, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dongbo LiuFengtian He
Sep 5, 2012·Chinese Journal of Cancer·Hui-Juan Yang
Jun 6, 2012·Journal of Virological Methods·Divya A PatelElectra D Paskett
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Apr 30, 2017·Cancer Biomarkers : Section a of Disease Markers·Arif AhmadSyed Akhtar Husain

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