p160 RhoA-binding kinase ROKalpha induces neurite retraction.

The Journal of Biological Chemistry
H KatohM Negishi

Abstract

We previously reported that the activation of prostaglandin E receptor EP3 subtype caused neurite retraction via small GTPase Rho in the EP3B receptor-expressing PC12 cells (Katoh, H., Negishi, M., and Ichikawa, A. (1996) J. Biol. Chem. 271, 29780-29784). However, a potential downstream effector of Rho that induces neurite retraction was not identified. Here we examined the morphological effect of p160 RhoA-binding kinase ROKalpha, a target for RhoA recently identified, on the nerve growth factor-differentiated PC12 cells. Microinjection of the catalytic domain of ROKalpha rapidly induced neurite retraction similar to that induced by microinjection of a constitutively active Rho, RhoV14, whereas microinjection of the kinase-deficient catalytic domain of ROKalpha did not induce neurite retraction. This morphological change was observed even though C3 exoenzyme, which was known to inactivate Rho, had been preinjected. On the other hand, microinjection of the Rho-binding domain or the pleckstrin homology domain of ROKalpha inhibited the EP3 receptor-induced neurite retraction. These results demonstrate that ROKalpha induces neurite retraction acting downstream of Rho in neuronal cells.

References

Jan 1, 1995·Methods in Enzymology·A J Self, A Hall
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Nov 22, 1996·The Journal of Biological Chemistry·H KatohA Ichikawa
Aug 1, 1996·Trends in Cell Biology·L M Machesky, A Hall

❮ Previous
Next ❯

Citations

Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Carina L BosHenri H Versteeg
Jun 14, 2005·Molecular and Cellular Neurosciences·Carlos Fernández-HernandoMiguel A Lasunción
Nov 14, 2000·Trends in Neurosciences·A Acebes, A Ferrús
Jul 24, 2002·Neuroscience Research·Yuko FukataKozo Kaibuchi
Jun 16, 2000·Advanced Drug Delivery Reviews·A M HopkinsA Nusrat
Nov 13, 2002·Neurochemistry International·Bor Luen Tang
Jan 13, 2000·Biophysical Chemistry·Y FukataK Kaibuchi
Feb 27, 1999·Current Opinion in Cell Biology·P Aspenström
May 14, 2003·Progress in Retinal and Eye Research·Stefan IsenmannAlessandro Cellerino
Jun 19, 2002·Biochimica Et Biophysica Acta·C L SayasF Wandosell
Jul 23, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dongsheng WuXuenong Bo
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N NakamuraK Kaibuchi
Oct 13, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y NakamuraM Takeda
Oct 23, 2002·Journal of Neurochemistry·Gary Meyer, Eva L Feldman
Feb 14, 2006·IEE Proceedings. Nanobiotechnology·P D Chantler, S R Wylie
Sep 10, 2003·Molecular Biology of the Cell·Steven R Wylie, Peter D Chantler
Mar 29, 2013·Molecular Biology of the Cell·Shuhei UedaHironori Katoh
Sep 13, 2000·Molecular and Cellular Biology·H KatohM Negishi
Mar 23, 2002·Molecular and Cellular Biology·Donghua TianSima Lev
Apr 10, 2002·Molecular and Cellular Biology·Hironori KatohManabu Negishi
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·R M BreyerM D Breyer
Sep 29, 2007·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·André SchmandkeStephen M Strittmatter
Jan 6, 2006·Biological & Pharmaceutical Bulletin·Weigang DuanMing Yan
Mar 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hitomi SannoKerry L Tucker
Jun 30, 2000·Biochemical and Biophysical Research Communications·T KanekoK Kaibuchi
Nov 10, 2000·Biochemical and Biophysical Research Communications·T IzawaK Kaibuchi
Apr 14, 2012·International Journal of Cell Biology·Mita VargheseCarol A Heckman
Sep 6, 2002·Pigment Cell Research·Glynis Scott
Sep 8, 2007·Canadian Journal of Physiology and Pharmacology·Pasquale ChitanoThomas M Murphy
Oct 3, 2002·The Journal of Cell Biology·Gianluca GalloPaul C Letourneau
Apr 20, 1999·The Journal of Cell Biology·Y FukataK Kaibuchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.