Abstract
The purpose of the present study was to identify the expression of p16INK4 in cervical cancer precursor lesions by immunohistochemistry and to correlate it with lesion grade and presence of human papillomavirus (HPV) infection. Cervical specimens from 144 women seen consecutively at the gynecology outpatient clinic of our institution from December 2003 to May 2005 were analyzed by cytopathology, histopathology, polymerase chain reaction for HPV-DNA, and p16INK4 immunostaining. Histologically normal biopsies, HPV-DNA negative by polymerase chain reaction, were used as control. HPV-DNA prevalence, including the control group, was 68.1% and the prevalence of p16INK4 expression was 55.0%. The percentage of cells stained by p16INK4 ranged from 10 to 100%, both in the group consisting of cervical intraepithelial neoplasia (CIN)1/HPV specimens and in the group of CIN2/CIN3 specimens with P value of 0.0001. p16INK4 expression was 48.3% in the CIN1/HPV group, as opposed to 94.3% in the CIN2/CIN3 group (P = 0.001), showing a statistically significant difference between the two groups. The quantitative method used here is simple and less subjective than the different semiquantitative methods described in the literature. In view of the dif...Continue Reading
References
Sep 20, 1995·Journal of the National Cancer Institute·M H Schiffman
Dec 6, 1996·Science·C J Sherr
Nov 1, 1996·Sexually Transmitted Diseases·N MuñozK V Shah
Dec 10, 1998·The American Journal of Pathology·T SanoT Nakajima
Jun 5, 1999·Carcinogenesis·L I Huschtscha, R R Reddel
Jun 17, 1999·Clinical Obstetrics and Gynecology·T V Sedlacek
Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Aug 24, 1999·American Journal of Obstetrics and Gynecology·R K Falls
Jan 5, 2000·Journal of Clinical Microbiology·P E GravittR J Apple
Apr 13, 2000·The Journal of Infection·S M CavalcantiL H Oliveira
Apr 14, 2000·American Journal of Obstetrics and Gynecology·W J LedgerS S Witkin
Aug 2, 2000·American Journal of Obstetrics and Gynecology·W M LinC Y Muller
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·R KlaesM von Knebel Doeberitz
Apr 20, 2001·Journal of Virology·M GiarrèM Tommasino
Jun 23, 2001·The American Journal of Surgical Pathology·J T KeatingC P Crum
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Jul 16, 2002·American Journal of Epidemiology·Yolanda van der GraafJan van den Tweel
Dec 25, 2002·Journal of Clinical Pathology·N MurphyJ J O'Leary
Jul 16, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·S Nicholas AgoffLaura A Koutsky
Jun 9, 2004·Journal of Clinical Microbiology·S-M KulmalaK Syrjänen
Sep 24, 2004·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·M BrancaK Syrjänen
Apr 16, 2005·The American Journal of Surgical Pathology·Alexandra N KalofKumarasen Cooper
Citations
Feb 2, 2010·Pediatrics International : Official Journal of the Japan Pediatric Society·Xiaohong YueZhengwei Yuan
Jul 22, 2014·The Journal of Pathology·Gordana HalecMichael Pawlita
Feb 9, 2010·The American Journal of Surgical Pathology·Alcides ChauxAntonio L Cubilla
Aug 8, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M O L P CostaM T S Correia
Nov 23, 2011·International Journal of Surgical Pathology·Julie A JacksonÇağatay Erşahin
Apr 30, 2021·Journal of Medical Virology·Wei LuPing Chen