P2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC

Journal of Bacteriology
Dmitry MarkovKonstantin Severinov

Abstract

Escherichia coli strain 397c carries a temperature-sensitive mutation, rpoC397, that removes the last 50 amino acids of the RNA polymerase beta' subunit and is nonpermissive for plating of bacteriophage P2. P2 gor mutants productively infect 397c and define a new gene, lysC, encoded by a reading frame that extensively overlaps the P2 lysis accessory gene, lysB. The unusual location of lysC with respect to lysB is reminiscent of the Rz/Rz1 lysis gene pair of phage lambda. Indeed, coexpression of lysB and lysC complemented the growth defect of lambda Rz/Rz1 null mutants, indicating that the LysB/C pair is similar to Rz/Rz1 in both gene arrangement and function. Cells carrying the rpoC397 mutation exhibited an early onset of P2-induced lysis, which was suppressed by the gor mutation in lysC. We propose that changes in host gene expression resulting from the rpoC397 mutation result in changes in the composition of the bacterial cell wall, making the cell more susceptible to P2-mediated lysis and preventing accumulation of progeny phage sufficient for plaque formation.

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Citations

Aug 21, 2012·Journal of Bacteriology·Joel BerryRy Young
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Oct 27, 2010·BMC Genomics·Karlene H LynchJonathan J Dennis
Oct 12, 2012·BMC Genomics·Jason J GillRy Young
May 5, 2016·Bacteriophage·Gail E Christie, Richard Calendar
Feb 1, 2012·Research in Microbiology·Jeong-A LimSangryeol Ryu
Sep 29, 2007·Journal of Molecular Biology·Elizabeth J SummerRy Young

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