P2 nucleotide receptors on C2C12 satellite cells.

Purinergic Signalling
Wiktor BanachewiczJolanta Barańska

Abstract

In developing muscle cells environmental stimuli transmitted by purines binding to the specific receptors are crucial proliferation regulators. C2C12 myoblasts express numerous purinergic receptors representing both main classes: P2X and P2Y. Among P2Y receptors we have found the expression of P2Y(1), P2Y(2), P2Y(4), P2Y(6) and P2Y(12) family members while among P2X receptors P2X(4), P2X(5) and P2X(7) were discovered. We have been able to show that activation of those receptors is responsible for ERK class kinase activity, responsible for regulation of cell proliferation pathway. We have also demonstrated that this activity is calcium dependent suggesting Ca(2+) ions as secondary messenger between receptor and kinase regulatory system. More specifically, we do suspect that in C2C12 myoblasts calcium channels of P2X receptors, particularly P2X(5) play the main role in proliferation regulation. In further development of myoblasts into myotubes, when proliferation is gradually inhibited, the pattern of P2 receptors is changed. This phenomenon is followed by diminishing of the P2Y(2)-dependent Ca(2+) signaling, while the mRNA expression of P2Y(2) receptor reminds still on the high level. Moreover, P2X(2) receptor mRNA, absent in my...Continue Reading

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Citations

May 2, 2012·Purinergic Signalling·Karina Kaczmarek-HájekAnnette Nicke
Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mariana Cintra-FrancischinelliCesare Montecucco
Aug 13, 2015·International Journal of Molecular Sciences·Yukako TokutakeShinichi Yonekura
Aug 25, 2012·Free Radical Biology & Medicine·Marina SciancaleporePaola Lorenzon
May 17, 2015·Autonomic Neuroscience : Basic & Clinical·Isabel R Orriss
Aug 22, 2013·Biochemical and Biophysical Research Communications·Mohammad A ChowdhuryGregory R Monteith
Feb 11, 2011·Molecular and Cellular Biochemistry·Tiziana MartinelloRomeo Betto
Jul 17, 2010·Journal of Applied Physiology·Chau-Zen WangChia-Hsin Chen
Aug 23, 2020·International Journal of Molecular Sciences·Chiara PanicucciClaudio Bruno
Feb 20, 2021·Pharmacology & Therapeutics·Aleksandra SzopaPiotr Wlaź

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