P2-purinoceptor-mediated membrane currents in DDT1 MF-2 smooth muscle cells.

European Journal of Pharmacology
A MollemanA Den Hertog

Abstract

The electrophysiological response evoked by ATP was investigated in the DDT1 MF-2 smooth muscle cell line using the microelectrode technique and the whole-cell patch clamp technique. Application of ATP (10(-3) M) to the bathing solution caused a small initial depolarization of the cell membrane, followed by hyperpolarization and slow depolarization. During voltage clamping (-50 mV) a triphasic response was recorded on stimulation with ATP (10(-4)-10(-3) M). A short-lasting inward current was followed by a transient outward current and a slowly decreasing inward current. This response was not affected by the receptor antagonists, propranolol (3 X 10(-6) M), phentolamine (3 X 10(-6) M), atropine (3 X 10(-6) M) or theophylline (10(-3) M). The ATP-induced currents were not modified by the voltage-dependent channel blocking agents, tetraethyl ammonium (3 X 10(-3) M), 3,4-diaminopyridine (10(-3) M), tetrodotoxin (3 X 10(-7) M) or diltiazem (10(-5) M). The fast inward current was not detectable at a low ATP concentration (10(-5) M). The outward current showed a reversal potential near -76 mV, which equals the potassium equilibrium potential. This current was abolished after neutralization of the potassium electrochemical gradient. The...Continue Reading

References

May 1, 1978·British Journal of Pharmacology·G BurnstockL Kasakov
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Jul 29, 1988·Biochemical and Biophysical Research Communications·D EscandeI Cavero
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Citations

Jan 1, 1991·Pflügers Archiv : European journal of physiology·A MollemanA den Hertog
May 14, 1992·European Journal of Pharmacology·L Van der ZeeA Den Hertog
Aug 1, 1992·British Journal of Pharmacology·R H HenningA den Hertog
Feb 17, 2001·The Journal of Physiology·M BeggA Molleman
Jan 22, 1998·The American Journal of Physiology·S D KohK M Sanders

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