P2 purinoceptor-mediated stimulation of adenylyl cyclase in PC12 cells

European Journal of Pharmacology
Y YakushiY Nomura

Abstract

PC12 pheochromocytoma cells have P2 purinoceptors which are activated by ATP and coupled to Ca2+ influx and catecholamine release. Also PC12 cells have adenosine receptors coupled positively to adenylyl cyclase, and cyclic AMP regulates cell functions such as catecholamine release. The effects of ATP and ATP analogs on cyclic AMP accumulation in PC12 cells were investigated in this study. ATP and adenosine 5'-0-(3-thiotriphosphate) stimulated cyclic AMP accumulation at low concentrations up to 300 microM but showed inhibitory effects above this concentration. 2',3'-O-(4-Benzoyl)benzoyl ATP and 2-methylthio ATP showed similar effects, although the responses were very limited. Addition of adenosine 5'-O-(2-thiodiphosphate) (ADP beta S) or beta, gamma-methylene ATP, but not alpha, beta-methylene ATP, stimulated cyclic AMP accumulation markedly without causing an inhibitory phase. The effects of ATP, ADP beta S and beta, gamma-methylene ATP were not inhibited by adenosine deaminase or specific antagonists to A1 and A2 adenosine receptors. Neither ADp beta S nor beta, gamma-methylene ATP showed any effect on Ca2+ influx or noradrenaline release. Suramin, a P2 receptors antagonists, had no inhibitory effect against ATP analog-stimula...Continue Reading

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Citations

Jul 3, 1998·European Journal of Pharmacology·T MurayamaY Nomura
Mar 22, 2000·British Journal of Pharmacology·S OhkuboI Matsuoka
Feb 9, 2002·British Journal of Pharmacology·Ursula UnterbergerStefan Boehm
Aug 6, 2005·Neurochemistry International·Katrin Sak, Peter Illes
Nov 26, 1998·Japanese Journal of Pharmacology·M P Abbracchio, G Burnstock
Oct 5, 2001·Journal of Cardiovascular Pharmacology and Therapeutics·M K ShahP J Kadowitz
Apr 7, 1999·Archives of Biochemistry and Biophysics·T NaganumaY Nomura

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