P2 purinoceptors in cultured bovine middle cerebral artery endothelial cells

Journal of Cardiovascular Pharmacology
H ZhangR L Macdonald

Abstract

Extracellular adenosine triphosphate (ATP) plays an important role in the regulation of endothelial function. However, its receptors and their signal-transduction pathways in major cerebral arterial endothelial cells are largely unknown. This study was undertaken functionally to classify the P2 purinoceptors in cultured bovine middle cerebral artery endothelial cells by using [Ca2+]i microfluorimetry. The rank order of potency to increase [Ca2+]i was 2-methylthio-ATP approximately ATP approximately uridine triphosphate (UTP) > adenosine diphosphate (ADP) > adenosine monophosphate (AMP) > alpha,beta-methylene-ATP > adenosine, suggesting that the effect was mediated by both P2y and P2u receptors. ATP, 2-methylthio-ATP, and UTP mobilized Ca2+ from intracellular stores and triggered Ca2+ entry. The effects of ATP, 2-methylthio-ATP, and UTP were reduced by phospholipase C inhibitor 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (NCDC), but only the effects of ATP and UTP were attenuated by pertussis toxin, indicating that P2y and P2u receptors may activate the same effector mechanisms by coupling to different G proteins. The [Ca2+]i entry caused by UTP was significantly reduced by the receptor-regulated Ca2+ channel blocker SK&F 9636...Continue Reading

References

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Citations

Dec 12, 2001·Experimental Gerontology·L Y MiaoJ H Zhang
Jun 9, 2006·Neurological Research·Robert P OstrowskiJohn H Zhang
Mar 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Yasushi Miyagi, John H Zhang
Feb 7, 2001·Stroke; a Journal of Cerebral Circulation·R C CarpenterJ H Zhang
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic

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