P2 receptor-mediated inhibition of adenylyl cyclase in PC12 cells

European Journal of Pharmacology
T MurayamaY Nomura

Abstract

PC12 pheochromocytoma cells have P2 receptors which are coupled to Ca2+ influx and catecholamine release. Previously we reported that ATP stimulated cyclic AMP accumulation at low concentrations up to 100 microM but showed inhibitory effects above this concentration [Yakushi, Y., Watanabe. A.. Murayama, T., Nomura, Y., 1996. Eur. J. Pharmacol. (314) 243-248]. In this study we investigated the characteristics of the inhibitory effects of ATP analogs. In the presence of 10 microM forskolin, an activator of adenylyl cyclase, ATP, adenosine 5'-O-(3-thiotriphosphate) (ATPgammaS), 2',3'-O-(4-benzoyl) benzoyl ATP, 2-methylthio ATP and adenosine 5'-O-(2-thiodiphosphate) inhibited cyclic AMP accumulation in a dose-dependent manner from 100 microM. UTP, alphabeta and betagamma-methylene ATP had no or very limited effects. The relative order of ATP analogs suggests that the ATP receptor appears to be P2Y-like. However, suramin, an antagonist of P2X and P2Y receptors, and reactive blue-2, which inhibited betagamma-methylene ATP-induced cyclic AMP accumulation, did not modify the inhibitory effect of ATPgammaS. Treatment with pertussis toxin, which completely abolished the effect of carbachol, had no effect on the action of ATP over 300 mic...Continue Reading

References

Feb 19, 1992·Biochimica Et Biophysica Acta·C el-MoatassimJ C Mani
Sep 9, 1992·Biochimica Et Biophysica Acta·M A MajidY Kondo
Sep 18, 1992·Cell·W J Tang, A G Gilman
Feb 1, 1992·British Journal of Pharmacology·S KeppensH De Wulf
Sep 15, 1989·Biochemical and Biophysical Research Communications·I PianetJ Labouesse
Jan 15, 1986·The Biochemical Journal·J L Gordon
Nov 3, 1995·The Journal of Biological Chemistry·K ChangY Takuwa
Jan 6, 1995·The Journal of Biological Chemistry·R Taussig, A G Gilman
Aug 31, 1993·Biochemical and Biophysical Research Communications·M A MajidY Kondo
Dec 29, 1995·The Journal of Biological Chemistry·D CommuniJ M Boeynaems
Feb 6, 1996·Biochemical and Biophysical Research Communications·T E WebbE A Barnard
Aug 2, 1996·The Journal of Biological Chemistry·G K AkbarS P Kunapuli
Dec 1, 1996·Journal of Cellular Physiology·T MurayamaY Nomura
Oct 24, 1996·European Journal of Pharmacology·Y YakushiY Nomura
Jan 1, 1997·Archives of Biochemistry and Biophysics·H OdaY Nomura
Jan 24, 1998·The Journal of Biological Chemistry·D CommuniJ M Boeynaems

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Citations

Mar 23, 2000·Neuropharmacology·N D'AmbrosiC Volonté
Feb 9, 2002·British Journal of Pharmacology·Ursula UnterbergerStefan Boehm
Aug 6, 2005·Neurochemistry International·Katrin Sak, Peter Illes
Sep 17, 2004·Journal of Neuroscience Research·Holger Peter Behrsing, P Richard Vulliet

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