p21 is required for atRA-mediated growth inhibition of MEPM cells, which involves RAR

Journal of Cellular Biochemistry
Zengli YuYingxin Pei

Abstract

All-trans retinoic acid (atRA), a metabolite of vitamin A, is essential for embryonic development. Thus the spatial and temporal dispersal of RA must be tightly controlled. Previous studies show that excessive atRA led to growth inhibition and p21 accumulation in mouse embryonic palatal mesenchymal (MEPM) cells. We reported here the identification of p21 as a required mediator during atRA-induced growth inhibition. atRA caused a G1 arrest in the cell cycle with an increase in the proportion of cells in G0/G1 and a decrease in the proportion of cells in S phase. In addition to a marked effect on cell cycling, atRA also triggered DNA fragmentation, reflected by an increase of the fraction of cells in the sub-G(1) population. Western blot analysis revealed that atRA treatment led to an increase in p21 level and a decrease in cyclin D1 protein and Rb phosphorylation. Using luciferase assay with reporter gene regulated by p21 promoter, we showed that atRA increased the reporter activity in a dose-dependent manner; and p21 siRNA blocked the growth inhibition by atRA, suggesting that p21 is required for atRA-mediated growth inhibition. Moreover, the induction of p21 by atRA was partially attenuated when RAR was silenced with specific ...Continue Reading

References

Oct 3, 1985·The New England Journal of Medicine·E J LammerI T Lott
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Feb 1, 1994·Mechanisms of Development·P DolléP Chambon
Sep 1, 1996·Pediatric Pathology & Laboratory Medicine : Journal of the Society for Pediatric Pathology, Affiliated with the International Paediatric Pathology Association·S CoberlyM Alashari
Aug 1, 1997·Molecular and Cellular Biology·V V OgryzkoB H Howard
Aug 6, 1998·Oral Oncology·M CardinaliW A Yeudall
Nov 21, 1998·Veterinary Pathology·K A Schafer
May 20, 1999·Annual Review of Pharmacology and Toxicology·M D Collins, G E Mao
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Jul 14, 2000·Physiological Reviews·S A RossL M De Luca
Nov 4, 2000·Cell Proliferation·M Molinari
Mar 10, 2001·The Journal of Nutrition·M H Zile
Apr 21, 2001·Oncogene·W R Taylor, G R Stark
Jun 5, 2001·Nature Reviews. Genetics·A O Wilkie, G M Morriss-Kay
May 29, 2002·Clinical Genetics·J C Murray
Jul 27, 2002·Annual Review of Pharmacology and Toxicology·Li-Na Wei
Jun 23, 2004·Development, Growth & Differentiation·Sussie DalvinT Bernard Kinane
Nov 13, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Zengli YuYong Li
Nov 30, 2005·Journal of Anatomy·Sylvia A HilliardYi Ping Chen
May 23, 2006·Toxicology and Applied Pharmacology·Zengli Yu, Ying Xing
Apr 20, 2007·Toxicology and Applied Pharmacology·Junko OkanoKohei Shiota
Aug 24, 2007·Development·Josephine Bowles, Peter Koopman

❮ Previous
Next ❯

Citations

Oct 16, 2012·Nature Structural & Molecular Biology·QiDong HuMichael G Rosenfeld
Jun 8, 2013·ILAR Journal·Ratnam S SeelanRobert M Greene
Aug 21, 2014·Oxidative Medicine and Cellular Longevity·Barbora BrodskáAleš Holoubek
Apr 24, 2009·Birth Defects Research. Part A, Clinical and Molecular Teratology·Miao WangYiyang Chen
Oct 25, 2016·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Huijing Wang, Weiliang Chen
Oct 6, 2020·Neurosurgery·Jeffrey I TraylorFranco DeMonte

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.