P21(Cip1/WAF1) expression in the mouse testis before and after X irradiation

Molecular Reproduction and Development
T L BeumerD G De Rooij

Abstract

During spermatogenesis, the radiosensitivity of testicular cells changes considerably. To investigate the molecular mechanisms underlying these radiosensitivity differences, p21(Cip1/WAF1) expression was studied before and after irradiation in the adult mouse testis. P21(Cip1/WAF1) is a cyclin-dependent kinase inhibitor (CDI) and has a role in the G1/S checkpoint and differentiation. P21(Cip1/WAF1) expression was observed in the normal testis, using Western blotting analysis. After a dose of 4 Gy, but not after 0.3 Gy, an increase in p21(Cip1/WAF1) expression could be determined in whole testis lysates. To investigate which germ cells are involved in p21(Cip1/WAF1) protein expression, immunohistochemical analysis was performed on irradiated testis. In the normal testis a weak staining for p21(Cip1/WAF1) was found in pachytene spermatocytes in epithelial stage V up to step 5 spermatids. A dose of 4 Gy of X-irradiation resulted in a transient increase of p21(Cip1/WAF1) staining in these cells with a maximum at 6 h post irradiation, despite the fact that the irradiation did not induce an increase in the number of apoptotic spermatocytes. When a dose of 0.3 Gy was given, no increase in p21(Cip1/WAF1) staining was observed. Using th...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J KuerbitzM B Kastan
Oct 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M BianchiJ H Hendry
Oct 12, 1995·Nature·J BrugarolasG J Hannon
Aug 11, 1995·The Journal of Biological Chemistry·M AkashiH P Koeffler
Mar 1, 1993·American Journal of Respiratory Cell and Molecular Biology·T P StrandjordD K Madtes
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Flores-RozasJ Hurwitz
Oct 1, 1994·International Journal of Radiation Biology·I Szumiel
Apr 1, 1994·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A MaityR J Muschel
Jul 1, 1993·Molecular and Cellular Biology·Q ZhanA J Fornace
Jan 1, 1993·Molecular and Cellular Biology·J J RyanM F Clarke
Jan 1, 1996·Genes & Development·E White

❮ Previous
Next ❯

Citations

Jun 22, 2000·Molecular Reproduction and Development·T L BeumerD G De Rooij
Jan 6, 2001·Molecular Reproduction and Development·A West, J Lähdetie
Jan 23, 1999·Current Opinion in Cell Biology·D G de Rooij, J A Grootegoed
Feb 25, 2005·Biology of Reproduction·Denise R HolsbergerPaul S Cooke
Feb 9, 2011·Molecular and Cellular Biology·Chamutal BornsteinVarda Rotter
Jun 9, 2005·Reproductive Biology and Endocrinology : RB&E·Anne DamestoyPhilippe Durand
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·M L WatsonR W Moreadith
Nov 26, 2003·International Journal of Andrology·Geert HamerDirk G De Rooij
Jun 26, 2012·Environmental and Molecular Mutagenesis·Eugenia CordelliPaola Villani
Oct 24, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J PerrinJ-L Bergé-Lefranc
Aug 16, 2005·The International Journal of Biochemistry & Cell Biology·Alessandra di PietroSteven de Jong
May 20, 2005·Differentiation; Research in Biological Diversity·Sanny S W ChungDebra J Wolgemuth
Jan 16, 2009·Biology of Reproduction·Anne ForandJacqueline Bernardino-Sgherri
Nov 26, 1999·Biology of Reproduction·J Blanco-Rodríguez, C Martínez-García
Apr 24, 2001·Environmental and Molecular Mutagenesis·A Ronen, B W Glickman
May 16, 2006·Molecular Reproduction and Development·Shannon Matulis, Mary Ann Handel
Mar 31, 1999·Endocrinology·T L BeumerD G de Rooij
May 20, 2003·The Journal of Pathology·Diana C J SpieringsSteven de Jong
Oct 20, 2012·Molecular Biology Reports·Dong-Song NieXiang Yang
Jan 21, 2003·Biology of Reproduction·Geert HamerDirk G de Rooij
Nov 7, 1998·International Journal of Andrology·J Blanco-Rodríguez
May 17, 2019·Journal of Toxicologic Pathology·Asako OkabeTetsuya Tsukamoto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.