P21waf-1/cip-1/sdi-1 is expressed at G1 phase in primary culture of hepatocytes from old rats, presumably preventing the cells from entering the S phase of the cell cycle

Biochemical and Biophysical Research Communications
N SawadaM Mori

Abstract

To elucidate whether p21waf-1/cip-1/sdi-1 expression is associated with loss of growth potential of hepatocytes of old rats, we determined p21waf-1/cip-1/sdi-1 expression of hepatocytes from old (30 months) rats during the cell cycle in primary culture. A high level of expression of p21waf-1/cip-1/sdi-1 was detected at the G1 phase in old-rat hepatocytes, but after the S phase in young-rat hepatocytes. Consistently, the incidence of the cells positive for p21waf-1/cip-1/sdi-1 in nuclei before entering the S phase was significantly higher in old-rat hepatocytes than in young-rat hepatocytes. These results account for the loss of growth potential of old-rat hepatocytes in vitro and the marked retardation of regeneration of liver in old rats in vivo.

Citations

Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G S Crary, J H Albrecht
Jun 17, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Zhaosheng LinMegan S Lim
Sep 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T KojimaD C Spray

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