p27 Kip1 nuclear localization and cyclin-dependent kinase inhibitory activity are regulated by glycogen synthase kinase-3 in human colon cancer cells.

Cell Death and Differentiation
Q WangB M Evers

Abstract

The cellular mechanisms regulating intestinal differentiation are poorly understood. Sodium butyrate (NaBT), a short-chain fatty acid, increases p27 Kip1 expression and induces cell cycle arrest associated with intestinal cell differentiation. Here, we show that treatment of intestinal-derived cells with NaBT induced G0/G1 arrest and intestinal alkaline phosphatase, a marker of differentiation, activity and mRNA expression; this induction was attenuated by inhibition of glycogen synthase kinase-3 (GSK-3). Moreover, treatment with NaBT increased the nuclear, but not the cytosolic, expression and activity of GSK-3beta. NaBT decreased cyclin-dependent kinase CDK2 activity and induced p27 Kip1 expression; inhibition of GSK-3 rescued NaBT-inhibited CDK2 activity and blocked NaBT-induced p27 Kip1 expression in the nucleus but not in the cytoplasm. In addition, we demonstrate that NaBT decreased the expression of S-phase kinase-associated protein 2 (Skp2), and this decrease was attenuated by GSK-3 inhibition. Furthermore, NaBT increased p27 Kip1 binding to CDK2, which was completely abolished by GSK-3 inhibition. Overexpression of an active form of GSK-3beta reduced Skp2 expression, increased p27 Kip1 in the nucleus and increased p27 ...Continue Reading

References

Jun 18, 1993·Cell·C J Sherr
Jun 2, 1997·The Journal of Cell Biology·A PtasznikA Hayek
Nov 14, 1998·The American Journal of Physiology·Q M DingB M Evers
Nov 26, 1998·Molecular and Cellular Neurosciences·E A MarcusW Harris
Apr 25, 2000·Molecular and Cellular Biology·E Santoni-RugiuJ Lukas
Sep 26, 2000·American Journal of Physiology. Cell Physiology·A QuaroniC Ap Rhys
Feb 13, 2001·Gastroenterology·C DeschênesN Rivard
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·C J Phiel, P S Klein
Mar 6, 2002·The Journal of Biological Chemistry·Elisheva SmithBaruch Frenkel
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Leonard J ApplemanVassiliki A Boussiotis
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Piyajit WatcharasitRichard S Jope
Oct 18, 2003·The Journal of Biological Chemistry·Mark A GregoryStephen R Hann
Jan 31, 2004·The Journal of Biological Chemistry·Patricia Rouet-BenzinebAndré Bado
Jul 16, 2005·International Journal of Radiation Biology·Céline HatonNina M Griffiths
Aug 17, 2005·Transfusion Medicine·D ChoD W Ryang
Feb 14, 2006·Cancer Cell·Andrew Koff
Aug 23, 2006·Journal of Cellular Biochemistry·Corinth A AuldRon F Morrison
Sep 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Inke TimmerbeulNisar P Malek
May 29, 2007·Cellular and Molecular Life Sciences : CMLS·J E Forde, T C Dale

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Citations

May 29, 2009·The Journal of Biological Chemistry·Abdullah YalcinJason Chesney
May 31, 2013·Carcinogenesis·Gérald BernatchezJulie C Carrier
May 24, 2011·PloS One·Qingding WangB Mark Evers
Nov 26, 2010·Science China. Life Sciences·Jia LiNing Li
Sep 22, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Yu-Jin ChoByung-Lan Lee
Aug 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jean-Marc BlouinSylvie Bortoli
Dec 19, 2015·Oxidative Medicine and Cellular Longevity·Adriana BorrielloFulvio Della Ragione
Sep 28, 2015·Seminars in Cancer Biology·Shahab UddinRamzi M Mohammad
Oct 14, 2009·PloS One·Serdar KorurMaria Maddalena Lino
Jun 15, 2010·Experimental Hematology·Emma Y SongAlla Dolnikov
Dec 10, 2016·Cell Death and Differentiation·Qingding WangB Mark Evers
Feb 18, 2016·PloS One·Heather Szabo-RogersKaren J Liu
Oct 17, 2018·The Biochemical Journal·Carrie DraneyJeffery S Tessem
Nov 10, 2017·Cancer Cell International·Wen-Ting WeiWen-Ting Liao

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