PMID: 8939353Oct 1, 1996Paper

P2u purinoceptor modulation of intracellular Ca2+ in a human lung adenocarcinoma cell line: down-regulation of Ca2+ influx by protein kinase C

Cell Calcium
M T Clunes, P J Kemp

Abstract

The human lung small cell adenocarcinoma cell line, A549, demonstrates a concentration-dependent rise in [Ca2+]i in response to extracellular nucleotides. The cells show Ca2+ mobilization on addition of various nucleotides, with an order of agonist potency: UTP > or = ATP > ADP > ADP beta S > AMP; adenosine is ineffective. The EC50 values for UTP and ATP are 12.5 +/- 0.4 microM and 18.9 +/- 0.5 microM, respectively. Together, these results are strongly indicative of the P2U subclass being the major nucleotide receptor expressed in these cells. The Ca2+ response was typically biphasic consisting of an initial spike, representing release of Ca2+ from internal stores, and a subsequent plateau representing Ca2+ influx. The majority of cells showed an agonist-induced Ca2+ increase that was unaffected by pretreatment with the Ca(2+)-ATPase inhibitors 2,5-di(tert-butyl)1,4-benzohydroquinone or thapsigargin. Caffeine did not raise [Ca2+]i above basal levels and applied in conjunction with nucleotide did not attenuate the agonist-mediated response. The Ca2+ influx was sensitive to protein kinase C, and agonist addition in the presence of a protein kinase C inhibitor, D-erythrosphingosine, produced a significantly potentiated Ca2+ influx...Continue Reading

References

Nov 13, 1995·Biochemical and Biophysical Research Communications·C SchöflS Wagner
Nov 1, 1994·The American Journal of Physiology·M J StuttsR C Boucher
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·A B Parekh, R Penner
Jan 1, 1995·American Journal of Respiratory Cell and Molecular Biology·W R RiceD T Fiedeldey
Jul 1, 1994·Physiological Reviews·T PozzanJ Meldolesi
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
Jan 28, 1993·Nature·M J Berridge
Mar 1, 1993·Trends in Pharmacological Sciences·V Sorrentino, P Volpe

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Citations

Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Feb 1, 1996·Digestive Diseases and Sciences·N CuiR N Fedorak
Aug 14, 2012·Pharmacological Reviews·Geoffrey BurnstockJean-Pierre Timmermans
Feb 7, 2001·The Journal of Clinical Endocrinology and Metabolism·C J TaiP C Leung
Jan 22, 1998·The American Journal of Physiology·D C MarcusM A Scofield
Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·D M ZhaoH Nakamura
May 14, 2021·Purinergic Signalling·M Reyna-JeldesF G Vázquez-Cuevas

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