P2X7 receptor cross-talk regulates ATP-induced pannexin 1 internalization

The Biochemical Journal
Andrew K J Boyce, Leigh Anne Swayne

Abstract

In the nervous system, extracellular ATP levels transiently increase in physiological and pathophysiological circumstances, effecting key signalling pathways in plasticity and inflammation through purinergic receptors. Pannexin 1 (Panx1) forms ion- and metabolite-permeable channels that mediate ATP release and are particularly enriched in the nervous system. Our recent study demonstrated that elevation of extracellular ATP triggers Panx1 internalization in a concentration- and time-dependent manner. Notably, this effect was sensitive to inhibition of ionotropic P2X7 purinergic receptors (P2X7Rs). Here, we report our novel findings from the detailed investigation of the mechanism underlying P2X7R-Panx1 cross-talk in ATP-stimulated internalization. We demonstrate that extracellular ATP triggers and is required for the clustering of P2X7Rs and Panx1 on Neuro2a cells through an extracellular physical interaction with the Panx1 first extracellular loop (EL1). Importantly, disruption of P2X7R-Panx1 clustering by mutation of tryptophan 74 within the Panx1 EL1 inhibits Panx1 internalization. Notably, P2X7R-Panx1 clustering and internalization are independent of P2X7R-associated intracellular signalling pathways (Ca(2+) influx and Src a...Continue Reading

References

Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
Apr 9, 2004·American Journal of Physiology. Cell Physiology·Jeffrey F Hiken, Thomas H Steinberg
Aug 12, 2004·FEBS Letters·Li BaoGerhard Dahl
Jul 20, 2005·The European Journal of Neuroscience·Arundhati RayRolf Dermietzel
Sep 7, 2005·Brain Research. Molecular Brain Research·Angelika VogtHannah Monyer
Jan 18, 2006·Journal of Lipid Research·Mikel Garcia-MarcosStéphanie Pochet
Oct 10, 2006·FEBS Letters·Ran Zalk, Varda Shoshan-Barmatz
May 5, 2007·Neuroscience·S McGaraughtyM F Jarvis
May 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pablo PelegrinAnnmarie Surprenant
Jul 4, 2008·American Journal of Physiology. Cell Physiology·R IglesiasE Scemes
Jul 24, 2008·Cell Communication & Adhesion·Daniela BoassaGina Sosinsky
Oct 24, 2008·American Journal of Physiology. Cell Physiology·Feng Qiu, Gerhard Dahl
Nov 15, 2008·Trends in Neurosciences·Maria P AbbracchioHerbert Zimmermann
Nov 22, 2008·The Journal of Pharmacology and Experimental Therapeutics·Weihong MaAnnmarie Surprenant
May 7, 2009·The Journal of Biological Chemistry·William R SilvermanGerhard Dahl
Aug 21, 2009·Molecular Biology of the Cell·Silvia PenuelaDale W Laird
Jun 10, 2010·The Journal of Biological Chemistry·Leigh Anne SwayneSteffany A L Bennett
Oct 12, 2010·Mass Spectrometry Reviews·Maroun Bou KhalilDaniel Figeys
Apr 13, 2011·Purinergic Signalling·Helio Miranda Costa-JuniorRobson Coutinho-Silva
Sep 13, 2011·Progress in Neurobiology·Geoffrey BurnstockPeter Illes
Sep 21, 2011·Journal of Molecular Neuroscience : MN·V PoornimaAmal Kanti Bera
Jan 24, 2012·Biochimica Et Biophysica Acta·Alberto Baroja-MazoPablo Pelegrín
Feb 9, 2012·The Journal of Biological Chemistry·Joanna K SandilosDouglas A Bayliss
Feb 24, 2012·Neurochemical Research·Rodolfo M Iglesias, David C Spray
Mar 31, 2012·Neural Development·Leigh E Wicki-StordeurLeigh Anne Swayne
Jan 4, 2013·PloS One·Nora ProchnowGeorg Zoidl
Dec 10, 2013·Frontiers in Cellular Neuroscience·Lucy E Robinson, Ruth D Murrell-Lagnado
Jan 15, 2014·Channels·Yu-Hsin ChiuDouglas A Bayliss
Feb 20, 2014·FEBS Letters·Alexander W Lohman, Brant E Isakson
Mar 1, 2014·Channels·Brant E Isakson, Roger J Thompson
Jun 4, 2014·Cold Spring Harbor Perspectives in Biology·Satyajit MayorJulie G Donaldson
Sep 17, 2014·Trends in Pharmacological Sciences·Beáta Sperlágh, Peter Illes
Oct 5, 2014·The Journal of Biological Chemistry·Lucy E RobinsonRuth D Murrell-Lagnado

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Citations

Jul 22, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Leon J DeLalioBrant E Isakson
Mar 1, 2019·The Journal of Biological Chemistry·Leon J DeLalioBrant E Isakson
Jan 25, 2019·Annual Review of Immunology·Joel LindenGerhard Dahl
Jun 11, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Leigh Anne SwayneBrant E Isakson
Apr 20, 2018·International Journal of Molecular Sciences·Michel DoschGuido Beldi
Mar 15, 2020·Neurochemical Research·Yisi ShanZhiwei Gao
Mar 13, 2020·Journal of Neurochemistry·Albert K YeungMichael F Jackson
Jun 14, 2020·Scientific Reports·Nickie SafarianGeorg Zoidl
Apr 25, 2020·Physiological Reviews·Christian GiaumeLuc Leybaert
Aug 24, 2019·Frontiers in Molecular Neuroscience·Robin KoppAnnette Nicke
Apr 2, 2020·Nature Structural & Molecular Biology·Zengqin DengPeng Yuan
Jan 5, 2021·Molecular and Cellular Biochemistry·Eijaz Ahmed Bhat, Nasreena Sajjad
Jan 28, 2021·International Journal of Molecular Sciences·Carolina Flores-MuñozAgustín D Martínez
Nov 30, 2019·Brain Research Bulletin·Wen-Jun ZhangZeng-Xu Liu
Feb 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wen-Jun ZhangHong-Liang Luo
Feb 23, 2021·Frontiers in Pharmacology·Anna Lisa GiulianiFrancesco Di Virgilio
Mar 7, 2021·International Journal of Molecular Sciences·Paloma A HarchaBrigitte van Zundert
Mar 24, 2021·Pharmacology & Therapeutics·Juan C Sanchez-AriasLeigh Anne Swayne
Jul 13, 2021·Purinergic Signalling·Michael KovalBrant E Isakson
Jul 9, 2020·ACS Chemical Neuroscience·Kathleen E NavisDarren J Derksen
Sep 4, 2021·Cell Calcium·Rutambhara Purohit, Amal Kanti Bera

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