P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation

International Journal of Molecular Medicine
Kuang PengPeng Qu

Abstract

Purinergic 2X7 receptor (P2X7R) and nucleotide‑binding oligomerization domain-like receptor protein 3 (NLRP3) are expressed in macrophages in atherosclerotic lesions. However, the mechanisms through which P2X7R participates in the inflammatory response in atherosclerosis remain largely unknown. The aim of the present study was to investigate the role of P2X7R in atherosclerosis and the mechanisms of action of the NLRP3 inflammasome following stimulation with oxidized low-density lipoprotein (oxLDL). We observed the expression and distribution of P2X7R in the atherosclerotic plaque in the coronary arteries from an autopsy specimen and in that of the aortic sinuses of apoE(-/-) mice by immunohistochemistry and immunofluorescence staining. The specificity of short interfering RNA (siRNA) was used to suppress P2X7R and NLRP3 mRNA expression. RT-qPCR and western blot analysis were used to analyze mRNA and protein expression, respectively. Co-immunoprecipitation was used to examine the interaction between protein kinase R (PKR) phosphorylation and NLRP3. P2X7R and NLRP3 were expressed at high levels in the atherosclerotic plaque in the coronary arteries. Stimulation with oxLDL upregulated P2X7R, NLRP3 and interleukin (IL)-1β expressi...Continue Reading

References

Oct 8, 2005·The Journal of Clinical Investigation·Adam E MullickLinda K Curtiss
Feb 20, 2010·Nature Reviews. Immunology·Jurg Tschopp, Kate Schroder
Oct 16, 2010·Lipids in Health and Disease·Luis Chávez-SánchezFrancisco Blanco-Favela
Apr 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katri NiemiKari K Eklund
Oct 13, 2011·Tissue Antigens·J S WileyS J Fuller
Apr 14, 2012·International Neurourology Journal·Chul-Su YangEun-Kyeong Jo
Jun 26, 2012·Developmental and Comparative Immunology·Jamal HussenHans-Joachim Schuberth
Jul 18, 2012·Nature·Ben LuKevin J Tracey
Jul 24, 2012·Biochemical and Biophysical Research Communications·Fumitake UsuiMasafumi Takahashi
Dec 15, 2012·Critical Reviews in Immunology·Kari K EklundP T Kovanen
Jan 15, 2013·Frontiers in Immunology·Aurélie GombaultIsabelle Couillin
Feb 16, 2013·Current Rheumatology Reports·Alena Grebe, Eicke Latz
Apr 23, 2013·International Journal of Cardiology·Mariarosaria PiscopielloChiara Foglieni
May 25, 2013·Nature Reviews. Immunology·Eicke LatzAndrea Stutz
Jun 27, 2013·The Journal of Biological Chemistry·Anne F McGettrick, Luke A J O'Neill
Jul 12, 2013·Mediators of Inflammation·Elisa BenettiMassimo Collino
Sep 24, 2013·Immunity·Haitao WenJenny P-Y Ting
Oct 8, 2013·Cellular & Molecular Immunology·Cécile Oury
Dec 10, 2013·Bioorganic & Medicinal Chemistry·Nisha MehtaOm Silakari
Feb 4, 2014·Human Immunology·Luis Chávez-SánchezFrancisco Blanco-Favela

❮ Previous
Next ❯

Citations

Nov 26, 2015·Frontiers in Pharmacology·Bo-Zong ShaoChong Liu
Sep 9, 2015·Frontiers in Immunology·Emilie VénéreauMarco Emilio Bianchi
Nov 11, 2017·Cardiovascular Research·Jack P GreenHeather L Wilson
Jun 23, 2016·Journal of Lipid Research·Meric Erikci Ertunc, Gökhan S Hotamisligil
Mar 28, 2017·Journal of Cellular Physiology·Zahra HoseiniHamed Mirzaei
Aug 4, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nikolett LénártÁdám Dénes
Jun 9, 2018·Circulation Research·Alena GrebeEicke Latz
May 22, 2016·Journal of the American Heart Association·Geena Paramel VargheseAllan Sirsjö
Nov 3, 2016·Circulation·Thiago Bruder-NascimentoRita C Tostes
Oct 26, 2016·Nature Communications·Min XieDaolin Tang
Oct 2, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Brandon G ShokoplesErnesto L Schiffrin
May 8, 2020·Clinical Reviews in Allergy & Immunology·Khadijeh KoushkiAmirhossein Sahebkar
Aug 8, 2020·Expert Opinion on Therapeutic Targets·Jeremiah StithamBabak Razani
Sep 29, 2020·Frontiers in Immunology·Tabassum RahmanJonathan A Harton
Dec 11, 2017·Basic Research in Cardiology·Dongling LiuXianwei Wang
Jun 1, 2018·Journal of Immunology Research·Wenyi ZhouYanfang Chen
Dec 14, 2018·Cell Proliferation·Zeng ZhaolinWang Zuo
Apr 21, 2019·Molecular Biology Reports·Yanji ZhuShuzhen Sun
Oct 5, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yusuke Hirata
Nov 22, 2019·Frontiers in Immunology·Ayesha ZahidJinhui Tao
Jun 26, 2017·Respiratory Research·Jie YinXiaolu Li
Jan 14, 2021·Cellular and Molecular Life Sciences : CMLS·Jie Wang AJunlian Gu
Oct 30, 2020·Journal of Inflammation Research·Meseret Derbew MollaTewodros Shibabaw
Feb 1, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xiao HeLiming Zhang
Apr 23, 2021·Circulation Journal : Official Journal of the Japanese Circulation Society·Masafumi Takahashi
Nov 14, 2020·International Journal of Molecular Sciences·María Ángeles Martínez-CuestaÁngeles Álvarez
May 18, 2021·Frontiers in Pharmacology·Junteng ZhouXin Cao
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Shanjun HuangZhouqing Huang
Oct 24, 2021·Experimental Hematology·Xiaoxiao HeJunke Zheng

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
PCR
protein assay
electrophoresis
transfection
co-immunoprecipitation

Software Mentioned

Adobe Photoshop
Pro Plus
SPSS
Image

Related Concepts

Related Feeds

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.