P2Y receptor regulation of cultured rat cerebral cortical cells: calcium responses and mRNA expression in neurons and glia

British Journal of Pharmacology
Gillian C BennettMichael R Boarder

Abstract

1 We have investigated increases in cytosolic Ca(2+) in response to nucleotides in mixed rat cerebrocortical cultures (neurons and glia in similar numbers) and in essentially neuron-free glial cultures. 2 In both cultures, the agonist-response profile was 2-methylthioADP(2MeSADP)>2-methylthioATP(2MeSATP)>ADP>ATP>adenosine 5'-O-(3-thiotriphosphate), consistent with a P2Y(1) receptor. The maximal responses to 2MeSADP, 2MeSATP and ADP were identical, but that to ATP was higher. 3 Suramin, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid, reactive blue 2 (RB2), and adenosine biphosphate (A3P5P) were antagonists with apparent pA(2) values of 5.5 for suramin, 6.4 for RB2, and 4.7 for A3P5P. 4 Single cell imaging divided the cells from the mixed neuronal-glial cultures into two populations: responsive (neurons) and unresponsive (glial cells) to high [K(+)]. The response of cells to nucleotides was almost exclusively limited to those not responsive to high K(+). 5 In the presence of extracellular Mn(2+), the response of the mixed cultures to 30 mM K(+) and 20 micro M Bay K 8644 was attenuated. However, when 2MeSADP was added there was no reduction in response in cultures previously loaded with Mn(2+). This further indicated that ...Continue Reading

References

Sep 1, 1988·Naunyn-Schmiedeberg's Archives of Pharmacology·K ShinozukaD P Westfall
May 1, 1995·British Journal of Pharmacology·I von KügelgenK Starke
Nov 1, 1994·British Journal of Pharmacology·I von KügelgenK Starke
Nov 12, 1993·Neuroscience Letters·G Bruner, S Murphy
Feb 3, 1994·European Journal of Pharmacology·C AllgaierG Hertting
May 1, 1996·British Journal of Pharmacology·J B SchachterT K Harden
Jun 1, 1996·British Journal of Pharmacology·S J CharltonM R Boarder
Oct 10, 1996·European Journal of Pharmacology·N S CheungP M Beart
Mar 1, 1997·Trends in Pharmacological Sciences·B B FredholmM Williams
Sep 23, 1997·British Journal of Pharmacology·S Koizumi, K Inoue
Nov 14, 1997·British Journal of Pharmacology·K NieberP Illes
Dec 24, 1997·Naunyn-Schmiedeberg's Archives of Pharmacology·G HansmannK Starke
May 19, 1998·Trends in Pharmacological Sciences·M R Boarder, S M Hourani
Jun 18, 1998·British Journal of Pharmacology·M S FaguraP Leff
Nov 7, 1998·Brain Research. Developmental Brain Research·U Lalo, P Kostyuk
Dec 1, 1999·British Journal of Pharmacology·D CommuniJ M Boeynaems
Dec 6, 2000·The Journal of Biological Chemistry·F L ZhangF J Monsma
Aug 1, 1976·Neuroscience·G Burnstock
Sep 8, 2001·The Journal of Biological Chemistry·D CommuniJ M Boeynaems
Feb 9, 2002·British Journal of Pharmacology·Ursula UnterbergerStefan Boehm
Apr 19, 2002·The Journal of Pharmacology and Experimental Therapeutics·Fang L ZhangFrederick Monsma
Feb 1, 2003·Trends in Pharmacological Sciences·Maria P AbbracchioGeoffrey Burnstock

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Citations

May 13, 2006·Pflügers Archiv : European journal of physiology·Simon Hussl, Stefan Boehm
Mar 14, 2009·Molecular Neurobiology·Alexei VerkhratskyGeoffrey Burnstock
Aug 5, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R R ResendeH Ulrich
Nov 30, 2005·Journal of Neurochemistry·Kerstin WirknerPeter Illes
Jul 4, 2006·The Journal of Pain : Official Journal of the American Pain Society·Irina VetterPeter John Cabot
Aug 17, 2005·Pharmacology & Therapeutics·Heike Franke, Peter Illes
Jul 1, 2006·Journal of Neuroscience Research·Joseph T Neary, Yuan Kang
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard

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