P2Y(1) and P2Y(12) receptor cross-talk in calcium signalling: Evidence from nonstarved and long-term serum-deprived glioma C6 cells.

Purinergic Signalling
Dorota SuplatJolanta Barańska

Abstract

The current work presents results of experiments on the calcium response evoked by the stimulation by extracellular nucleotides occurring in control, nonstarved glioma C6 cells and in cells after long-term (96 h) serum starvation. Three nucleotide receptors were studied: P2Y(1), P2Y(2) and P2Y(12). Two of them, P2Y(1) and P2Y(2), directly stimulate calcium response. The protein level of the P2Y(2) receptor did not change during the serum starvation, while P2Y(1) protein level fell dramatically. Observed changes in the calcium response generated by P2Y(1) are directly correlated with the receptor protein level as well as with the amount of calcium present in the intracellular calcium stores, partially depleted during starvation process. The third receptor, P2Y(12), did not directly evoke calcium response, however it is activated by the same ligand as P2Y(1). The experiments with AR-C69941MX, the P2Y(12)-specific antagonist, indicated that in control and serum-starved cells, calcium response evoked by P2Y(1) receptor is potentiated by the activity of P2Y(12)-dependent signaling pathways. This potentiation may be mediated by P2Y(12) inhibitory effect on the plasma membrane calcium pump. The calcium influx enhanced by the cooperati...Continue Reading

References

Feb 1, 1986·Cell Calcium·J W Putney
Nov 15, 1995·The Biochemical Journal·M J Berridge
Jan 1, 1994·Pharmacology & Therapeutics·M P Abbracchio, G Burnstock
Jan 27, 1995·Cell·D E Clapham
Mar 1, 1994·Trends in Pharmacological Sciences·C FasolatoT Pozzan
Oct 1, 1993·Endocrine Reviews·J W Putney, G S Bird
Jan 28, 1993·Nature·M J Berridge
Mar 21, 1998·The Journal of Biological Chemistry·Y S BaeS G Rhee
Mar 11, 2000·Trends in Pharmacological Sciences·J M BoeynaemsJ M Herbert
Apr 14, 2004·Current Topics in Medicinal Chemistry·Peter Illes, Joaquim A Ribeiro
Aug 6, 2005·Neurochemistry International·Katrin Sak, Peter Illes
Feb 13, 2007·Brain Research Bulletin·Patryk KrzeminskiJolanta Brańska

❮ Previous
Next ❯

Citations

Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Jul 22, 2008·European Journal of Pharmacology·Patryk KrzemińskiJolanta Barańska
Mar 27, 2018·British Journal of Pharmacology·J J MicklewrightS J Fountain

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
fluorescence microscopy
glycosylation

Software Mentioned

MS Excel
Ingenious station

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.