P2y1 and P2y2 receptor-operated Ca2+ signals in primary cultures of cardiac microvascular endothelial cells

Microvascular Research
Francesco MocciaFranco Tanzi

Abstract

Intracellular Ca2+ signals elicited by nucleotide agonists were investigated in primary cultures of rat cardiac microvascular endothelial cells using the fura-2 technique. UTP increased the intracellular [Ca2+] in 94% of the cells, whereas 2MeSATP was active in 32%. The rank order of potency was ATP = UTP > 2MeSATP and the maximal response to 2MeSATP was lower compared to UTP and ATP. ATP and UTP showed strong homologous and heterologous desensitization. ATP fully inhibited the 2MeSATP response, while UTP abolished 2MeSATP-elicited transients in 25% of cells. 2MeSATP did not desensitize the UTP or ATP response. Adenosine 2',5'-diphosphate inhibited the response to 2MeSATP, while it did not modify the response to ATP and UTP. 2MeSATP was more sensitive to suramin than UTP and ATP. These results indicate that P(2Y1) and P(2Y2) receptors may be coexpressed in CMEC. Nucleotide-induced Ca2+ signals lacked a sustained plateau and were almost independent from extracellular Ca2+. ATP and UTP elicited Ca2+ transients longer than 2MeSATP-evoked transients. The kinetics of Ca2+ responses was not affected by bath solution stirring or ectonucleotidase inhibition. Furthermore, the nonhydrolyzable ATP analogue AMP-PNP induced Ca2+ signals sim...Continue Reading

References

Apr 1, 1991·Trends in Pharmacological Sciences·S E O'ConnorP Leff
Jan 1, 1990·Trends in Pharmacological Sciences·J M Boeynaems, J D Pearson
Jan 15, 1986·The Biochemical Journal·J L Gordon
Nov 1, 1993·British Journal of Pharmacology·A J Vials, G Burnstock
Apr 1, 1996·British Journal of Pharmacology·S YangM E Bradley
Jun 1, 1996·British Journal of Pharmacology·S J CharltonM R Boarder
Mar 11, 2000·Trends in Pharmacological Sciences·J M BoeynaemsJ M Herbert
Apr 12, 2000·European Journal of Biochemistry·C DangelmaierS P Kunapuli
Jul 20, 2000·Journal of Molecular and Cellular Cardiology·F MocciaF Tanzi

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Citations

Aug 4, 2010·Stem Cells and Development·Yuly Sánchez-HernándezFrancesco Moccia
Sep 24, 2011·Journal of Applied Physiology·Shawn B BenderJohnathan D Tune
May 17, 2012·PloS One·Abdul Q SheikhDaria A Narmoneva
Aug 21, 2012·World Journal of Biological Chemistry·Francesco MocciaFranco Tanzi
Oct 22, 2013·Heart and Vessels·Bao-Hai ZhangXiang-Jun Zeng
Jun 24, 2008·Endothelium : Journal of Endothelial Cell Research·Priscila SanabriaFernando A Gonzalez
Mar 22, 2007·British Journal of Pharmacology·C L M SilvaR P Markus
Dec 21, 2014·Purinergic Signalling·Geoffrey Burnstock, Amir Pelleg
Mar 22, 2018·International Journal of Molecular Sciences·Germano GuerraFrancesco Moccia
May 14, 2008·International Heart Journal·Willmann LiangXiaodong Wang
Jun 26, 2008·Journal of Vascular Research·Roberto Berra RomaniFranco Tanzi
Jun 5, 2019·Cancers·Giorgia ScarpellinoLuca Munaron
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
Aug 8, 2015·Journal of Cellular Physiology·Francesco Moccia, Germano Guerra
Sep 27, 2003·American Journal of Physiology. Heart and Circulatory Physiology·F MocciaD J Adams
Feb 9, 2021·Frontiers in Physiology·Francesco MocciaFrancesco Lodola
Sep 3, 2002·Microvascular Research·Francesco MocciaFranco Tanzi

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