P2Y13 and P2X7 receptors modulate mechanically induced adenosine triphosphate release from mast cells.

Experimental Dermatology
Dan ShenLina Wang

Abstract

Subcutaneous mast cells (MCs) are vulnerable to mechanical stimulation from external environment. Thus, MCs immune function could be modulated by their mechanosensitivity. This property has been identified as the trigger mechanism of needling acupuncture, a traditional oriental therapy. Previously we have demonstrated the release of adenosine triphosphate (ATP), a stress-responsive signalling molecule, from mechanical-perturbed MCs. The current work explores its underlying mechanisms. We noticed that propagation of intracellular free Ca2+ occurred among HMC-1 cells in response to 50% hypotonic shock. Additionally, amplifying cascade of ATP-induced ATP release was observed in RBL-2H3 cells stimulated by medium displacement, which could be mimicked by exogenous ATP (exoATP). Secondary ATP liberation induced by low level (50 nmol/L) of exoATP was reduced by inhibiting ecto-ATPase-dependent ADP production with ARL67156, or blocking P2 receptors with suramin or PPADS, or with specific P2Y13 receptor antagonist MRS2211, or siRNA. Secondary ATP release induced by higher dose (200 μmol/L) of exoATP, sufficient to stimulate P2X7 receptor, was attenuated by suramin, PPADS or specific P2X7 receptor antagonist BBG, or siRNA. Finally, RT-PC...Continue Reading

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Citations

Jun 25, 2020·Purinergic Signalling·Jin-Rong HePeter Illes
Oct 10, 2020·Purinergic Signalling·Yong Tang, Peter Illes
Dec 18, 2020·Frontiers in Cellular Neuroscience·Dilyara NurkhametovaRashid Giniatullin
Mar 2, 2021·The American Journal of Chinese Medicine·Zhi-Ying LvLei-Miao Yin
Jun 3, 2021·Journal of Clinical Medicine·Thomas PerreaultBarry C Gendron

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