P2Y2 receptor promotes intestinal microtubule stabilization and mucosal re-epithelization in experimental colitis

Journal of Cellular Physiology
Emilie DegagnéFernand-Pierre Gendron

Abstract

P2Y(2) receptor expression is increased in intestinal epithelial cells (IECs) during inflammatory bowel diseases (IBDs). In this context, P2Y(2) stimulates PGE(2) release by IECs, suggesting a role in wound healing. For this study, we have used the non-cancerous IEC-6 cell line. IEC-6 cell migration was determined using Boyden chambers and the single-edged razor blade model of wounding. The receptor was activated using ATP, UTP, or 2-thioUTP. Pharmacological inhibitors, a blocking peptide, a neutralizing antibody and interfering RNAs were used to characterize the signaling events. Focal adhesions and microtubule (MT) dynamics were determined by immunofluorescence using anti-vinculin and anti-acetylated-α-tubulin antibodies, respectively. In vivo, the dextran sodium sulfate mouse model of colitis was used to characterize the effects of P2Y(2) agonist 2-thioUTP on remission. We showed that P2Y(2) increased cell migration and wound closure by recruiting Go protein with the cooperation of integrin α(v) . Following P2Y(2) activation, we demonstrated that GSK3β activity was inhibited in response to Akt activation. This leads to MT stabilization and increased number of focal adhesions. In vivo, P2Y(2) activation stimulates remission, ...Continue Reading

References

Jan 15, 1986·The Biochemical Journal·J L Gordon
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G G Gundersen, J C Bulinski
Jul 14, 1998·The Journal of Cell Biology·I KaverinaJ V Small
Sep 3, 1998·European Journal of Clinical Investigation·A U DignassH Goebell
Dec 9, 1998·Clinical and Experimental Immunology·L A DielemanE P Van Rees
Aug 24, 1999·British Journal of Pharmacology·E R Lazarowski, T K Harden
Feb 5, 2000·Advances in Experimental Medicine and Biology·R D Berg
Feb 17, 2000·Journal of the National Cancer Institute·H J AgtereschJ H Wilson
Jun 6, 2000·Molecular and Cellular Biochemistry·B VelázquezF A González
Mar 10, 2001·Inflammatory Bowel Diseases·A U Dignass
Oct 3, 2002·Gastroenterology·Michael JeffersHenri S Lichenstein
Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·Bernhard Wehrle-Haller, Beat A Imhof
Mar 12, 2003·Journal of Leukocyte Biology·Andrea la SalaGiampiero Girolomoni
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·Douglas Taupin, Daniel K Podolsky
Jun 5, 2004·Gastroenterology Clinics of North America·Kim L Isaacs, R Balfour Sartor
Aug 4, 2004·Cell and Tissue Research·D T LuttikhuizenM J A van Luyn
Sep 27, 2005·Digestive Diseases and Sciences·Ryuichi Okamoto, Mamoru Watanabe
Sep 28, 2005·The Journal of Biological Chemistry·Sriparna BagchiLaurie Erb
Apr 6, 2006·Pflügers Archiv : European journal of physiology·Laurie ErbGary A Weisman
Apr 25, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Michael BraunManfred Kietzmann
Nov 7, 2006·Current Biology : CB·Nathan A ReedKristen J Verhey
Dec 23, 2006·Journal of Cellular Physiology·Nataliya E ChornaFernando A González
Mar 16, 2007·Journal of Cell Science·Joseph M SchoberGary G Borisy
Apr 13, 2007·Physiological Reviews·Anthony T BlikslagerJack Odle
Jan 18, 2008·World Journal of Gastroenterology : WJG·Andreas Sturm, Axel U Dignass
Jan 18, 2008·World Journal of Gastroenterology : WJG·Mike G LaukoetterAsma Nusrat
Feb 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Djordje M GrbicFernand-Pierre Gendron
Mar 3, 2009·Inflammatory Bowel Diseases·Hideo Yoshida, D Neil Granger
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Margaret L GardelClare M Waterman
Jul 25, 2009·Current Opinion in Pharmacology·Francesco Di VirgilioSimon C Robson
Jul 28, 2009·European Journal of Immunology·Christine Langlois, Fernand-Pierre Gendron
Sep 8, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emilie DegagnéFernand-Pierre Gendron
Oct 20, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Mahnaz Fatahzadeh
Nov 7, 2009·Development, Growth & Differentiation·Tong SunLeung Kim
Nov 20, 2009·The New England Journal of Medicine·Clara Abraham, Judy H Cho
Dec 2, 2009·Nature Reviews. Gastroenterology & Hepatology·Guillaume Pineton de ChambrunJean-Frédéric Colombel
Dec 17, 2009·Gastroenterology Nursing : the Official Journal of the Society of Gastroenterology Nurses and Associates·Yvonne Leahy
Dec 25, 2009·Current Opinion in Cell Biology·Sandrine Etienne-Manneville
Apr 30, 2010·American Journal of Physiology. Cell Physiology·Ilene BoucherVickery Trinkaus-Randall

❮ Previous
Next ❯

Citations

Apr 25, 2014·American Journal of Physiology. Cell Physiology·Farid G El-SayedGary A Weisman
Jun 30, 2012·The FEBS Journal·Emilie DegagnéFernand-Pierre Gendron
Aug 15, 2013·British Journal of Pharmacology·Tatsuro NakamuraHiroshi Ozaki
Apr 2, 2020·Expert Opinion on Drug Discovery·Diego Dal BenRosaria Volpini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.