PMID: 8973570Dec 1, 1996Paper

p30, a novel protein target of mouse calcyclin (S100A6)

The Biochemical Journal
A Filipek, U Wojda

Abstract

A novel protein target of mouse calcyclin (S100A6) was detected by a gel overlay method with 125I-labelled calcyclin. Interaction of calcyclin with its 30 kDa target protein (p30) present in Ehrlich ascites tumour (EAT) cells depended on the presence of Ca2+ ions. The binding of p30, evidenced by the reaction with 125I-labelled calcyclin, was found to be of higher affinity than the binding between mouse calcyclin and annexin II or glyceraldehyde-3-phosphate dehydrogenase. Examination of tissue extracts by the gel overlay method has shown that p30 is present not only in the EAT cells but also in mouse brain and spleen. This novel target protein of mouse calcyclin was purified to homogeneity from EAT cells by means of Phenyl-Sepharose chromatography, affinity chromatography and CM-cellulose chromatography. Purified p30 was digested with alpha-chymotrypsin and a partial amino acid sequence of one of the resulting peptides was established. A database search analysis revealed that the sequence is unique, with a similarity of less than 55% to any other known protein sequence.

Citations

Jul 8, 2000·The Journal of Biological Chemistry·M NowotnyJ Kuznicki
Apr 3, 2002·The Journal of Biological Chemistry·Anna FilipekJacek Kuznicki
May 11, 2006·The Biochemical Journal·Liliana Santamaria-KisielGary S Shaw
Jul 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jie LiangDaiming Fan
Feb 14, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaoxuan NingShiren Sun
May 15, 2003·The Journal of Biological Chemistry·Marcin NowotnyJacek Kuznicki
Sep 18, 2008·The FEBS Journal·Naofumi MiwaSatoru Kawamura
Aug 29, 2012·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Ewa KilańczykAnna Filipek
Apr 7, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ewa KilanczykAnna Filipek
Mar 7, 2013·Apoptosis : an International Journal on Programmed Cell Death·Xiong ChenXuenong Ouyang
Aug 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B JastrzebskaJ Kúznicki
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Jan 23, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Agnieszka M Topolska-WośAnna Filipek
Apr 18, 2014·International Journal of Molecular Sciences·Bo FangJason H Huang
May 29, 2018·Journal of Cellular Physiology·Sara Rosińska, Anna Filipek
Sep 21, 2018·Frontiers in Cellular and Infection Microbiology·Zhili ChuZengqi Yang
Jun 5, 2020·International Journal of Molecular Sciences·Anna Filipek, Wiesława Leśniak
Jun 4, 2002·The Journal of Biological Chemistry·Anna FilipekJacek Kuznicki
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Jul 20, 2010·Biochimica Et Biophysica Acta·Gabriela SchneiderAnna Filipek

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